A human-mouse somatic hybrid line selected for human deoxycytidine deaminase

Teh sheng Chan, Cedric Long, Howard Green

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Abstract

A new selective medium has been developed for cells containing the enzyme deoxycytidine deaminase. This medium contains hypoxanthine, aminopterin, and 5-methyldeoxycytidine (HAM medium). To survive in the presence of the aminopterin, the cells must utilize deoxycytidine deaminase to convert the 5-methyldeoxycytidine to thymidine. The cells must also have thymidine kinase and hypoxanthine phosphoribosyltransferase. A mouse cell line deficient in deoxycytidine deaminase has been isolated from a deoxycytidine kinase-deficient line, using 5-bromodeoxycytidine as the selective agent. A hybrid line between this double mutant and a human diploid fibroblast was isolated in HAM medium. The hybrid line contains the chromosomes expected of a human-mouse hybrid. The deoxycytidine deaminase isozyme patterns on cellogel show that the human-mouse hybrid cell line produces an enzyme with an electrophoretic mobility intermediate between that of the human and that of the mouse.

Original languageEnglish (US)
Pages (from-to)81-90
Number of pages10
JournalSomatic Cell Genetics
Volume1
Issue number1
DOIs
StatePublished - Jan 1 1975

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ASJC Scopus subject areas

  • Genetics

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