### Abstract

We present a new probability-based method for protein identification using tandem mass spectra and protein databases. The method employs a hypergeometric distribution to model frequencies of matches between fragment ions predicted for peptide sequences with a specific (M + H)^{+} value (at some mass tolerance) in a protein sequence database and an experimental tandem mass spectrum. The hypergeometric distribution constitutes null hypothesis - all peptide matches to a tandem mass spectrum are random. It is used to generate a score characterizing the randomness of a database sequence match to an experimental tandem mass spectrum and to determine the level of significance of the null hypothesis. For each tandem mass spectrum and database search, a peptide is identified that has the least probability of being a random match to the spectrum and the corresponding level of significance of the null hypothesis is determined. To check the validity of the hypergeometric model in describing fragment ion matches, we used Χ^{2} test. The distribution of frequencies and corresponding hypergeometric probabilities are generated for each tandem mass spectrum. No proteolytic cleavage specificity is used to create the peptide sequences from the database. We do not use any empirical probabilities in this method. The scores generated by the hypergeometric model do not have a significant molecular weight bias and are reasonably independent of database size. The approach has been implemented in a database search algorithm, PEP_PROBE. By using a large set of tandem mass spectra derived from a set of peptides created by digestion of a collection of known proteins using four different proteases, a false positive rate of 5% is demonstrated.

Original language | English (US) |
---|---|

Pages (from-to) | 3792-3798 |

Number of pages | 7 |

Journal | Analytical Chemistry |

Volume | 75 |

Issue number | 15 |

DOIs | |

State | Published - Aug 1 2003 |

Externally published | Yes |

### Fingerprint

### ASJC Scopus subject areas

- Analytical Chemistry

### Cite this

**A hypergeometric probability model for protein identification and validation using tandem mass spectral data and protein sequence databases.** / Sadygov, Rovshan; Yates, John R.

Research output: Contribution to journal › Article

*Analytical Chemistry*, vol. 75, no. 15, pp. 3792-3798. https://doi.org/10.1021/ac034157w

}

TY - JOUR

T1 - A hypergeometric probability model for protein identification and validation using tandem mass spectral data and protein sequence databases

AU - Sadygov, Rovshan

AU - Yates, John R.

PY - 2003/8/1

Y1 - 2003/8/1

N2 - We present a new probability-based method for protein identification using tandem mass spectra and protein databases. The method employs a hypergeometric distribution to model frequencies of matches between fragment ions predicted for peptide sequences with a specific (M + H)+ value (at some mass tolerance) in a protein sequence database and an experimental tandem mass spectrum. The hypergeometric distribution constitutes null hypothesis - all peptide matches to a tandem mass spectrum are random. It is used to generate a score characterizing the randomness of a database sequence match to an experimental tandem mass spectrum and to determine the level of significance of the null hypothesis. For each tandem mass spectrum and database search, a peptide is identified that has the least probability of being a random match to the spectrum and the corresponding level of significance of the null hypothesis is determined. To check the validity of the hypergeometric model in describing fragment ion matches, we used Χ2 test. The distribution of frequencies and corresponding hypergeometric probabilities are generated for each tandem mass spectrum. No proteolytic cleavage specificity is used to create the peptide sequences from the database. We do not use any empirical probabilities in this method. The scores generated by the hypergeometric model do not have a significant molecular weight bias and are reasonably independent of database size. The approach has been implemented in a database search algorithm, PEP_PROBE. By using a large set of tandem mass spectra derived from a set of peptides created by digestion of a collection of known proteins using four different proteases, a false positive rate of 5% is demonstrated.

AB - We present a new probability-based method for protein identification using tandem mass spectra and protein databases. The method employs a hypergeometric distribution to model frequencies of matches between fragment ions predicted for peptide sequences with a specific (M + H)+ value (at some mass tolerance) in a protein sequence database and an experimental tandem mass spectrum. The hypergeometric distribution constitutes null hypothesis - all peptide matches to a tandem mass spectrum are random. It is used to generate a score characterizing the randomness of a database sequence match to an experimental tandem mass spectrum and to determine the level of significance of the null hypothesis. For each tandem mass spectrum and database search, a peptide is identified that has the least probability of being a random match to the spectrum and the corresponding level of significance of the null hypothesis is determined. To check the validity of the hypergeometric model in describing fragment ion matches, we used Χ2 test. The distribution of frequencies and corresponding hypergeometric probabilities are generated for each tandem mass spectrum. No proteolytic cleavage specificity is used to create the peptide sequences from the database. We do not use any empirical probabilities in this method. The scores generated by the hypergeometric model do not have a significant molecular weight bias and are reasonably independent of database size. The approach has been implemented in a database search algorithm, PEP_PROBE. By using a large set of tandem mass spectra derived from a set of peptides created by digestion of a collection of known proteins using four different proteases, a false positive rate of 5% is demonstrated.

UR - http://www.scopus.com/inward/record.url?scp=0043064042&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0043064042&partnerID=8YFLogxK

U2 - 10.1021/ac034157w

DO - 10.1021/ac034157w

M3 - Article

C2 - 14572045

AN - SCOPUS:0043064042

VL - 75

SP - 3792

EP - 3798

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 15

ER -