A kunitz protease inhibitor from dermacentor variabilis, a vector for spotted fever group rickettsiae, limits rickettsia montanensis invasion

Shane M. Ceraul, Ashley Chung, Khandra T. Sears, Vsevolod Popov, Magda Beier-Sexton, M. Sayeedur Rahman, Abdu F. Azad

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

A defining facet of tick-Rickettsia symbioses is the molecular strategy employed by each partner to ensure its own survival. Ticks must control rickettsial colonization to avoid immediate death. In the current study, we show that rickettsial abundance in the tick midgut increases once the expression of a Kunitz-type serine protease inhibitor from the American dog tick (Dermacentor variabilis) (DvKPI) is suppressed by small interfering RNA (siRNA). A series of in vitro invasion assays suggested that DvKPI limits rickettsial colonization during host cell entry. Interestingly, we observed that DvKPI associates with rickettsiae in vitro as well as in the tick midgut. Collectively, our data demonstrate that DvKPI limits host cell invasion by Rickettsia montanensis, possibly through an association with the bacterium.

Original languageEnglish
Pages (from-to)321-329
Number of pages9
JournalInfection and Immunity
Volume79
Issue number1
DOIs
StatePublished - Jan 2011

Fingerprint

Dermacentor
Rickettsia
Ticks
Protease Inhibitors
Fever
Tick Control
Serine Proteinase Inhibitors
Symbiosis
Small Interfering RNA
Dogs
Bacteria
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

A kunitz protease inhibitor from dermacentor variabilis, a vector for spotted fever group rickettsiae, limits rickettsia montanensis invasion. / Ceraul, Shane M.; Chung, Ashley; Sears, Khandra T.; Popov, Vsevolod; Beier-Sexton, Magda; Rahman, M. Sayeedur; Azad, Abdu F.

In: Infection and Immunity, Vol. 79, No. 1, 01.2011, p. 321-329.

Research output: Contribution to journalArticle

Ceraul, Shane M. ; Chung, Ashley ; Sears, Khandra T. ; Popov, Vsevolod ; Beier-Sexton, Magda ; Rahman, M. Sayeedur ; Azad, Abdu F. / A kunitz protease inhibitor from dermacentor variabilis, a vector for spotted fever group rickettsiae, limits rickettsia montanensis invasion. In: Infection and Immunity. 2011 ; Vol. 79, No. 1. pp. 321-329.
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