A liquid chromatography method with single quadrupole mass spectrometry for quantitative determination of indomethacin in maternal plasma and urine of pregnant patients

Xiao-Ming Wang, Daria I. Vernikovskaya, Tatiana Nanovskaya, Erik Rytting, Gary Hankins, Mahmoud Ahmed

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A liquid chromatography with single quadrupole mass spectrometry method was developed for the quantitative determination of indomethacin in the maternal plasma and urine of pregnant patients under treatment. A deuterium-labeled isotope of indomethacin (d4-indomethacin) was used as an internal standard. The maternal plasma and urine samples were acidified with 1.0M HCl then extracted with chloroform to achieve the extraction recovery range of 94-104% with variation less than 11%. Chromatographic separation was achieved by a Waters Symmetry C18 column with isocratic elution of 0.05% (v/v) formic acid aqueous solution and acetonitrile (47:53, v/v). An in-source fragmentation was applied on the single quadrupole mass spectrometer equipped with an electrospray ionization source at positive mode. The LC-ESI-MS quantification was performed in the selected ion monitoring mode targeting ions at m/z 139 for indomethacin and m/z 143 for its internal standard. The calibration curves were linear in the concentration ranges between 14.8 and 2.97×103ng/mL for plasma samples and between 10.5 and 4.21×103ng/mL for urine samples. The relative standard deviation of this method was less than 8% for intra- and inter-day assays, and the accuracy ranged between 90% and 108%.

Original languageEnglish (US)
Pages (from-to)123-128
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume78-79
DOIs
StatePublished - May 5 2013

Fingerprint

Liquid chromatography
Indomethacin
Liquid Chromatography
Mass spectrometry
Mass Spectrometry
Mothers
Urine
Plasmas
formic acid
Ions
Electrospray ionization
Deuterium
Mass spectrometers
Ion sources
Chloroform
Isotopes
Calibration
Assays
Recovery
Water

Keywords

  • Indomethacin
  • LC-MS
  • Method validation
  • Plasma
  • Urine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Spectroscopy
  • Clinical Biochemistry

Cite this

@article{75664837c4674b0e9f2b67d93c2e9864,
title = "A liquid chromatography method with single quadrupole mass spectrometry for quantitative determination of indomethacin in maternal plasma and urine of pregnant patients",
abstract = "A liquid chromatography with single quadrupole mass spectrometry method was developed for the quantitative determination of indomethacin in the maternal plasma and urine of pregnant patients under treatment. A deuterium-labeled isotope of indomethacin (d4-indomethacin) was used as an internal standard. The maternal plasma and urine samples were acidified with 1.0M HCl then extracted with chloroform to achieve the extraction recovery range of 94-104{\%} with variation less than 11{\%}. Chromatographic separation was achieved by a Waters Symmetry C18 column with isocratic elution of 0.05{\%} (v/v) formic acid aqueous solution and acetonitrile (47:53, v/v). An in-source fragmentation was applied on the single quadrupole mass spectrometer equipped with an electrospray ionization source at positive mode. The LC-ESI-MS quantification was performed in the selected ion monitoring mode targeting ions at m/z 139 for indomethacin and m/z 143 for its internal standard. The calibration curves were linear in the concentration ranges between 14.8 and 2.97×103ng/mL for plasma samples and between 10.5 and 4.21×103ng/mL for urine samples. The relative standard deviation of this method was less than 8{\%} for intra- and inter-day assays, and the accuracy ranged between 90{\%} and 108{\%}.",
keywords = "Indomethacin, LC-MS, Method validation, Plasma, Urine",
author = "Xiao-Ming Wang and Vernikovskaya, {Daria I.} and Tatiana Nanovskaya and Erik Rytting and Gary Hankins and Mahmoud Ahmed",
year = "2013",
month = "5",
day = "5",
doi = "10.1016/j.jpba.2013.02.006",
language = "English (US)",
volume = "78-79",
pages = "123--128",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
issn = "0731-7085",
publisher = "Elsevier",

}

TY - JOUR

T1 - A liquid chromatography method with single quadrupole mass spectrometry for quantitative determination of indomethacin in maternal plasma and urine of pregnant patients

AU - Wang, Xiao-Ming

AU - Vernikovskaya, Daria I.

AU - Nanovskaya, Tatiana

AU - Rytting, Erik

AU - Hankins, Gary

AU - Ahmed, Mahmoud

PY - 2013/5/5

Y1 - 2013/5/5

N2 - A liquid chromatography with single quadrupole mass spectrometry method was developed for the quantitative determination of indomethacin in the maternal plasma and urine of pregnant patients under treatment. A deuterium-labeled isotope of indomethacin (d4-indomethacin) was used as an internal standard. The maternal plasma and urine samples were acidified with 1.0M HCl then extracted with chloroform to achieve the extraction recovery range of 94-104% with variation less than 11%. Chromatographic separation was achieved by a Waters Symmetry C18 column with isocratic elution of 0.05% (v/v) formic acid aqueous solution and acetonitrile (47:53, v/v). An in-source fragmentation was applied on the single quadrupole mass spectrometer equipped with an electrospray ionization source at positive mode. The LC-ESI-MS quantification was performed in the selected ion monitoring mode targeting ions at m/z 139 for indomethacin and m/z 143 for its internal standard. The calibration curves were linear in the concentration ranges between 14.8 and 2.97×103ng/mL for plasma samples and between 10.5 and 4.21×103ng/mL for urine samples. The relative standard deviation of this method was less than 8% for intra- and inter-day assays, and the accuracy ranged between 90% and 108%.

AB - A liquid chromatography with single quadrupole mass spectrometry method was developed for the quantitative determination of indomethacin in the maternal plasma and urine of pregnant patients under treatment. A deuterium-labeled isotope of indomethacin (d4-indomethacin) was used as an internal standard. The maternal plasma and urine samples were acidified with 1.0M HCl then extracted with chloroform to achieve the extraction recovery range of 94-104% with variation less than 11%. Chromatographic separation was achieved by a Waters Symmetry C18 column with isocratic elution of 0.05% (v/v) formic acid aqueous solution and acetonitrile (47:53, v/v). An in-source fragmentation was applied on the single quadrupole mass spectrometer equipped with an electrospray ionization source at positive mode. The LC-ESI-MS quantification was performed in the selected ion monitoring mode targeting ions at m/z 139 for indomethacin and m/z 143 for its internal standard. The calibration curves were linear in the concentration ranges between 14.8 and 2.97×103ng/mL for plasma samples and between 10.5 and 4.21×103ng/mL for urine samples. The relative standard deviation of this method was less than 8% for intra- and inter-day assays, and the accuracy ranged between 90% and 108%.

KW - Indomethacin

KW - LC-MS

KW - Method validation

KW - Plasma

KW - Urine

UR - http://www.scopus.com/inward/record.url?scp=84874768791&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874768791&partnerID=8YFLogxK

U2 - 10.1016/j.jpba.2013.02.006

DO - 10.1016/j.jpba.2013.02.006

M3 - Article

VL - 78-79

SP - 123

EP - 128

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

ER -