A localized pattern photobleaching method for the concurrent analysis of rapid and slow diffusion processes

D. E. Koppel, Michael Sheetz

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A scanning pattern photobleaching method for the analysis of lateral transport is described and discussed. Fluorescence bleaching with a localized pattern allows for the concurrent analysis of motions over two very different characteristic distances: xi 0(-1), the repeat distance of the pattern, and W, the linear dimension of the illuminated region. The former motion is deduced from the decay of the modulation amplitude (of period xi 0(-1) of fluorescence scans with the attenuated pattern, the latter from the recovery of the average fluorescence intensity. Such analysis should prove useful for the study of samples with a wide range of diffusion coefficients, and for the separation of effects arising from lateral diffusion and association dynamics. Theoretical analyses are presented for three related problems: (a) the effect of pattern localization on the decay of the modulation amplitude, (b) the effect of the pattern modulation on the recovery of the average local fluorescence intensity, and (c) the effect of a limited diffusion space (with linear dimensions of only a few pattern periods) on the decay of the modulation amplitude.

Original languageEnglish (US)
Pages (from-to)175-181
Number of pages7
JournalBiophysical Journal
Volume43
Issue number2
DOIs
StatePublished - Jan 1 1983
Externally publishedYes

Fingerprint

Photobleaching
Fluorescence

ASJC Scopus subject areas

  • Biophysics

Cite this

A localized pattern photobleaching method for the concurrent analysis of rapid and slow diffusion processes. / Koppel, D. E.; Sheetz, Michael.

In: Biophysical Journal, Vol. 43, No. 2, 01.01.1983, p. 175-181.

Research output: Contribution to journalArticle

@article{7fc15cd97c754a1d9829c08d4e9e4b71,
title = "A localized pattern photobleaching method for the concurrent analysis of rapid and slow diffusion processes",
abstract = "A scanning pattern photobleaching method for the analysis of lateral transport is described and discussed. Fluorescence bleaching with a localized pattern allows for the concurrent analysis of motions over two very different characteristic distances: xi 0(-1), the repeat distance of the pattern, and W, the linear dimension of the illuminated region. The former motion is deduced from the decay of the modulation amplitude (of period xi 0(-1) of fluorescence scans with the attenuated pattern, the latter from the recovery of the average fluorescence intensity. Such analysis should prove useful for the study of samples with a wide range of diffusion coefficients, and for the separation of effects arising from lateral diffusion and association dynamics. Theoretical analyses are presented for three related problems: (a) the effect of pattern localization on the decay of the modulation amplitude, (b) the effect of the pattern modulation on the recovery of the average local fluorescence intensity, and (c) the effect of a limited diffusion space (with linear dimensions of only a few pattern periods) on the decay of the modulation amplitude.",
author = "Koppel, {D. E.} and Michael Sheetz",
year = "1983",
month = "1",
day = "1",
doi = "10.1016/S0006-3495(83)84338-0",
language = "English (US)",
volume = "43",
pages = "175--181",
journal = "Biophysical Journal",
issn = "0006-3495",
publisher = "Biophysical Society",
number = "2",

}

TY - JOUR

T1 - A localized pattern photobleaching method for the concurrent analysis of rapid and slow diffusion processes

AU - Koppel, D. E.

AU - Sheetz, Michael

PY - 1983/1/1

Y1 - 1983/1/1

N2 - A scanning pattern photobleaching method for the analysis of lateral transport is described and discussed. Fluorescence bleaching with a localized pattern allows for the concurrent analysis of motions over two very different characteristic distances: xi 0(-1), the repeat distance of the pattern, and W, the linear dimension of the illuminated region. The former motion is deduced from the decay of the modulation amplitude (of period xi 0(-1) of fluorescence scans with the attenuated pattern, the latter from the recovery of the average fluorescence intensity. Such analysis should prove useful for the study of samples with a wide range of diffusion coefficients, and for the separation of effects arising from lateral diffusion and association dynamics. Theoretical analyses are presented for three related problems: (a) the effect of pattern localization on the decay of the modulation amplitude, (b) the effect of the pattern modulation on the recovery of the average local fluorescence intensity, and (c) the effect of a limited diffusion space (with linear dimensions of only a few pattern periods) on the decay of the modulation amplitude.

AB - A scanning pattern photobleaching method for the analysis of lateral transport is described and discussed. Fluorescence bleaching with a localized pattern allows for the concurrent analysis of motions over two very different characteristic distances: xi 0(-1), the repeat distance of the pattern, and W, the linear dimension of the illuminated region. The former motion is deduced from the decay of the modulation amplitude (of period xi 0(-1) of fluorescence scans with the attenuated pattern, the latter from the recovery of the average fluorescence intensity. Such analysis should prove useful for the study of samples with a wide range of diffusion coefficients, and for the separation of effects arising from lateral diffusion and association dynamics. Theoretical analyses are presented for three related problems: (a) the effect of pattern localization on the decay of the modulation amplitude, (b) the effect of the pattern modulation on the recovery of the average local fluorescence intensity, and (c) the effect of a limited diffusion space (with linear dimensions of only a few pattern periods) on the decay of the modulation amplitude.

UR - http://www.scopus.com/inward/record.url?scp=0020507112&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020507112&partnerID=8YFLogxK

U2 - 10.1016/S0006-3495(83)84338-0

DO - 10.1016/S0006-3495(83)84338-0

M3 - Article

C2 - 6616006

AN - SCOPUS:0020507112

VL - 43

SP - 175

EP - 181

JO - Biophysical Journal

JF - Biophysical Journal

SN - 0006-3495

IS - 2

ER -