A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome

A potential homologue of the yeast PRP8 protein

Mariano Garcia-Blanco, Gordon J. Anderson, Jean Beggs, Phillip A. Sharp

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A mammalian protein of approximately 220 kDa (p220) was UV-crosslinked to precursor mRNAs (pre-mRNAs) under splicing conditions. The kinetics and biochemical requirements of the UV-crosslinking of p220 corresponded to the kinetics and biochemical requirements of spliceosome formation. On Western blots, antibodies against the yeast splicing factor PRP8 recognized a doublet of proteins, the faster migrating of which comigrated with p220. Furthermore, UV-crosslinked p220 was immunoprecipitated with anti-PRP8 antisera. These results suggest structural conservation of the splicing factor PRP8 from yeast to mammals and show that this protein is in close proximity to the pre-mRNA in the spliceosome.

Original languageEnglish (US)
Pages (from-to)3082-3086
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number8
StatePublished - Apr 1990
Externally publishedYes

Fingerprint

Spliceosomes
Fungal Proteins
RNA Precursors
Yeasts
Proteins
Immune Sera
Mammals
Western Blotting
Antibodies
RNA Splicing Factors

Keywords

  • Pre-mRNA splicing
  • UV-crosslinking

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome : A potential homologue of the yeast PRP8 protein. / Garcia-Blanco, Mariano; Anderson, Gordon J.; Beggs, Jean; Sharp, Phillip A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 8, 04.1990, p. 3082-3086.

Research output: Contribution to journalArticle

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