A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome: A potential homologue of the yeast PRP8 protein

Mariano A. Garcia-Blanco, Gordon J. Anderson, Jean Beggs, Phillip A. Sharp

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

A mammalian protein of approximately 220 kDa (p220) was UV-crosslinked to precursor mRNAs (pre-mRNAs) under splicing conditions. The kinetics and biochemical requirements of the UV-crosslinking of p220 corresponded to the kinetics and biochemical requirements of spliceosome formation. On Western blots, antibodies against the yeast splicing factor PRP8 recognized a doublet of proteins, the faster migrating of which comigrated with p220. Furthermore, UV-crosslinked p220 was immunoprecipitated with anti-PRP8 antisera. These results suggest structural conservation of the splicing factor PRP8 from yeast to mammals and show that this protein is in close proximity to the pre-mRNA in the spliceosome.

Original languageEnglish (US)
Pages (from-to)3082-3086
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number8
DOIs
StatePublished - Apr 1990
Externally publishedYes

Keywords

  • Pre-mRNA splicing
  • UV-crosslinking

ASJC Scopus subject areas

  • General

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