A mass spectrometric "Western blot" to evaluate the correlations between histone methylation and histone acetylation

Kangling Zhang, Joseph S. Siino, Patrick R. Jones, Peter M. Yau, E. Morton Bradbury

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Histone acetylation, methylation, and phosphorylation occur predominantly in the unstructured N-terminal domains or histone "tails". These modifications and others comprise a "histone code" that directly facilitates or antagonizes association of regulatory proteins with nucleosomes to mediate changes in chromatin structure and activity. Methylation of histone H3 outside of the tail region at lysine 79 has been reported for a variety of species ranging from yeast to humans and in some gene-specific cases appears to be associated with active chromatin and transcription. Whether methylation of lysine 79 is associated with other posttranslational modifications of the H3 tail is unknown. Using mass spectrometric relative quantitation, a mass spectrometric "Western blot", we compare methylation at lysines 4, 9, and 79 with acetylation of human histone H3. We find that the total levels of lysine 4 and 79 methylation (combined mono-, di-, and trimethylation) in the H3 population increase with the degree of H3 tail acetylation. The total amount of lysine 4 methylation increases progressively from less than 10% in the nonacetylated H3 to greater than 90% in the penta-acetylated H3. In addition, significant levels of lysine 4 trimethylation also occur in combination with the penta-acetylated H3 species. In contrast, the level of H3 lysine 9 trimethylation is greatest for the monoacetylated species while H3 lysine 9 acetylation occurs predominantly in hyper-acetylated (tetra- and penta-acetylated) H3 isoforms. Together, these results indicate that methylation of lysine 4 and 79 as well as the switch from lysine 9 methylation to acetylation are coordinated synchronously with H3 hyperacetylation as marks of active chromatin.

Original languageEnglish (US)
Pages (from-to)3765-3775
Number of pages11
JournalProteomics
Volume4
Issue number12
DOIs
StatePublished - Dec 2004
Externally publishedYes

Fingerprint

Acetylation
Methylation
Histones
Lysine
Western Blotting
Rubiaceae
Tail
Chromatin
Histone Code
Phosphorylation
Nucleosomes
Transcription
Post Translational Protein Processing
Yeast
Protein Isoforms
Genes
Yeasts
Switches
Association reactions

Keywords

  • Histone acetylation
  • Histone methylation
  • Mass spectrometry

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

A mass spectrometric "Western blot" to evaluate the correlations between histone methylation and histone acetylation. / Zhang, Kangling; Siino, Joseph S.; Jones, Patrick R.; Yau, Peter M.; Bradbury, E. Morton.

In: Proteomics, Vol. 4, No. 12, 12.2004, p. 3765-3775.

Research output: Contribution to journalArticle

Zhang, Kangling ; Siino, Joseph S. ; Jones, Patrick R. ; Yau, Peter M. ; Bradbury, E. Morton. / A mass spectrometric "Western blot" to evaluate the correlations between histone methylation and histone acetylation. In: Proteomics. 2004 ; Vol. 4, No. 12. pp. 3765-3775.
@article{e5f1ed1fa8d84e8e8c1add9819d7daa4,
title = "A mass spectrometric {"}Western blot{"} to evaluate the correlations between histone methylation and histone acetylation",
abstract = "Histone acetylation, methylation, and phosphorylation occur predominantly in the unstructured N-terminal domains or histone {"}tails{"}. These modifications and others comprise a {"}histone code{"} that directly facilitates or antagonizes association of regulatory proteins with nucleosomes to mediate changes in chromatin structure and activity. Methylation of histone H3 outside of the tail region at lysine 79 has been reported for a variety of species ranging from yeast to humans and in some gene-specific cases appears to be associated with active chromatin and transcription. Whether methylation of lysine 79 is associated with other posttranslational modifications of the H3 tail is unknown. Using mass spectrometric relative quantitation, a mass spectrometric {"}Western blot{"}, we compare methylation at lysines 4, 9, and 79 with acetylation of human histone H3. We find that the total levels of lysine 4 and 79 methylation (combined mono-, di-, and trimethylation) in the H3 population increase with the degree of H3 tail acetylation. The total amount of lysine 4 methylation increases progressively from less than 10{\%} in the nonacetylated H3 to greater than 90{\%} in the penta-acetylated H3. In addition, significant levels of lysine 4 trimethylation also occur in combination with the penta-acetylated H3 species. In contrast, the level of H3 lysine 9 trimethylation is greatest for the monoacetylated species while H3 lysine 9 acetylation occurs predominantly in hyper-acetylated (tetra- and penta-acetylated) H3 isoforms. Together, these results indicate that methylation of lysine 4 and 79 as well as the switch from lysine 9 methylation to acetylation are coordinated synchronously with H3 hyperacetylation as marks of active chromatin.",
keywords = "Histone acetylation, Histone methylation, Mass spectrometry",
author = "Kangling Zhang and Siino, {Joseph S.} and Jones, {Patrick R.} and Yau, {Peter M.} and Bradbury, {E. Morton}",
year = "2004",
month = "12",
doi = "10.1002/pmic.200400819",
language = "English (US)",
volume = "4",
pages = "3765--3775",
journal = "Proteomics",
issn = "1615-9853",
publisher = "Wiley-VCH Verlag",
number = "12",

}

TY - JOUR

T1 - A mass spectrometric "Western blot" to evaluate the correlations between histone methylation and histone acetylation

AU - Zhang, Kangling

AU - Siino, Joseph S.

AU - Jones, Patrick R.

AU - Yau, Peter M.

AU - Bradbury, E. Morton

PY - 2004/12

Y1 - 2004/12

N2 - Histone acetylation, methylation, and phosphorylation occur predominantly in the unstructured N-terminal domains or histone "tails". These modifications and others comprise a "histone code" that directly facilitates or antagonizes association of regulatory proteins with nucleosomes to mediate changes in chromatin structure and activity. Methylation of histone H3 outside of the tail region at lysine 79 has been reported for a variety of species ranging from yeast to humans and in some gene-specific cases appears to be associated with active chromatin and transcription. Whether methylation of lysine 79 is associated with other posttranslational modifications of the H3 tail is unknown. Using mass spectrometric relative quantitation, a mass spectrometric "Western blot", we compare methylation at lysines 4, 9, and 79 with acetylation of human histone H3. We find that the total levels of lysine 4 and 79 methylation (combined mono-, di-, and trimethylation) in the H3 population increase with the degree of H3 tail acetylation. The total amount of lysine 4 methylation increases progressively from less than 10% in the nonacetylated H3 to greater than 90% in the penta-acetylated H3. In addition, significant levels of lysine 4 trimethylation also occur in combination with the penta-acetylated H3 species. In contrast, the level of H3 lysine 9 trimethylation is greatest for the monoacetylated species while H3 lysine 9 acetylation occurs predominantly in hyper-acetylated (tetra- and penta-acetylated) H3 isoforms. Together, these results indicate that methylation of lysine 4 and 79 as well as the switch from lysine 9 methylation to acetylation are coordinated synchronously with H3 hyperacetylation as marks of active chromatin.

AB - Histone acetylation, methylation, and phosphorylation occur predominantly in the unstructured N-terminal domains or histone "tails". These modifications and others comprise a "histone code" that directly facilitates or antagonizes association of regulatory proteins with nucleosomes to mediate changes in chromatin structure and activity. Methylation of histone H3 outside of the tail region at lysine 79 has been reported for a variety of species ranging from yeast to humans and in some gene-specific cases appears to be associated with active chromatin and transcription. Whether methylation of lysine 79 is associated with other posttranslational modifications of the H3 tail is unknown. Using mass spectrometric relative quantitation, a mass spectrometric "Western blot", we compare methylation at lysines 4, 9, and 79 with acetylation of human histone H3. We find that the total levels of lysine 4 and 79 methylation (combined mono-, di-, and trimethylation) in the H3 population increase with the degree of H3 tail acetylation. The total amount of lysine 4 methylation increases progressively from less than 10% in the nonacetylated H3 to greater than 90% in the penta-acetylated H3. In addition, significant levels of lysine 4 trimethylation also occur in combination with the penta-acetylated H3 species. In contrast, the level of H3 lysine 9 trimethylation is greatest for the monoacetylated species while H3 lysine 9 acetylation occurs predominantly in hyper-acetylated (tetra- and penta-acetylated) H3 isoforms. Together, these results indicate that methylation of lysine 4 and 79 as well as the switch from lysine 9 methylation to acetylation are coordinated synchronously with H3 hyperacetylation as marks of active chromatin.

KW - Histone acetylation

KW - Histone methylation

KW - Mass spectrometry

UR - http://www.scopus.com/inward/record.url?scp=10644260359&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10644260359&partnerID=8YFLogxK

U2 - 10.1002/pmic.200400819

DO - 10.1002/pmic.200400819

M3 - Article

VL - 4

SP - 3765

EP - 3775

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 12

ER -