A membrane-bound human placental protein kinase activated by endogenous polypeptides

Mossaad Abdel-Ghany, Shun Nakamura, Javier Navarro, Efraim Racker

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A protein kinase (PPdPK) was purified from plasma membranes of human placenta. Phosphorylation of casein, but not of phosvitin or lactalbumin, by [γ-32P]ATP in the presence of PPdPK was stimulated about 10-fold by naturally occurring polypeptides prepared from avariety of sources similar to the procedure of Roberts et al. (Proc. Natl. Acad. Sci. U.S.A. 77, 3494-3498, 1980). The amino acid phos-phorylated on casein was serine. According to gel exclusion chromatography the mol.wt, of PPdPK was 95 000. In autoradiograms, following polyacrylamide-gel electrophoresis, the autophosphorylation of PPdPK was greatly enhanced by the polypeptide activators.

Original languageEnglish (US)
Pages (from-to)275-282
Number of pages8
JournalBioscience Reports
Volume3
Issue number3
DOIs
StatePublished - Mar 1983
Externally publishedYes

Fingerprint

Pregnancy Proteins
Caseins
Protein Kinases
Gel Chromatography
Phosvitin
Membranes
Lactalbumin
Peptides
Phosphorylation
Cell membranes
Chromatography
Electrophoresis
Serine
Placenta
Polyacrylamide Gel Electrophoresis
Adenosine Triphosphate
Gels
Cell Membrane
Amino Acids
polyacrylamide gels

ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

A membrane-bound human placental protein kinase activated by endogenous polypeptides. / Abdel-Ghany, Mossaad; Nakamura, Shun; Navarro, Javier; Racker, Efraim.

In: Bioscience Reports, Vol. 3, No. 3, 03.1983, p. 275-282.

Research output: Contribution to journalArticle

Abdel-Ghany, Mossaad ; Nakamura, Shun ; Navarro, Javier ; Racker, Efraim. / A membrane-bound human placental protein kinase activated by endogenous polypeptides. In: Bioscience Reports. 1983 ; Vol. 3, No. 3. pp. 275-282.
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