A method for measurement of free and bound plasma cyst(e)ine

Michael H. Malloy; David K. Rassin; Gerald E. Gaull

doi:10.1016/0003-2697(81)90095-6

A method for measurement of free and bound plasma cyst(e)ine

Michael H. Malloy, David K. Rassin, Gerald E. Gaull

Research output: Contribution to journal › Article › peer-review

87 Scopus citations

Abstract

A method for the measurement of free and bound cyst(e)ine and the sum thereof has been developed. In adult human blood, cyst(e)ine is distributed equally between that which is bound to plasma proteins and that which is free. Cyst(e)ine is bound predominantly to albumin, and this binding is not an in vitro artifact. Cysteine bound to plasma proteins may be displaced by homocysteine which competes for the available sulfhydryl groups of plasma proteins. Rats starved for 8 days had a significant decrease in both plasma free cyst(e)ine and bound cysteine. These data suggest that present methods for the determination of plasma cyst(e)ine under-estimate the quantity of cyst(e)ine in the plasma available for cellular metabolism.

Original language	English (US)
Pages (from-to)	407-415
Number of pages	9
Journal	Analytical Biochemistry
Volume	113
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(81)90095-6
State	Published - May 15 1981
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(81)90095-6

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title = "A method for measurement of free and bound plasma cyst(e)ine",

abstract = "A method for the measurement of free and bound cyst(e)ine and the sum thereof has been developed. In adult human blood, cyst(e)ine is distributed equally between that which is bound to plasma proteins and that which is free. Cyst(e)ine is bound predominantly to albumin, and this binding is not an in vitro artifact. Cysteine bound to plasma proteins may be displaced by homocysteine which competes for the available sulfhydryl groups of plasma proteins. Rats starved for 8 days had a significant decrease in both plasma free cyst(e)ine and bound cysteine. These data suggest that present methods for the determination of plasma cyst(e)ine under-estimate the quantity of cyst(e)ine in the plasma available for cellular metabolism.",

author = "Malloy, {Michael H.} and Rassin, {David K.} and Gaull, {Gerald E.}",

note = "Funding Information: Supported in part by the New York State Office of Mental Retardation and Developmental Disabilities and NIH Grant AM 20236. Dr. Malloy worked in this lab- oratory during the tenure of fellowship under National Research Service Award HD 07000 administered by the Institute of Human Nutrition of Columbia University. The authors are grateful for the expert technical assistance of Ms. Alice Gargano and secretarial assistance of Mrs. Lee Antonucci. We thank Dr. William Heird of Columbia University for helpful discussion during the course of the work as well as Professor Stanford Moore of Rockefeller University and Dr. M. K. Gaitonde of St. George{\textquoteright}s Hospital Medical School for their critical readings of the manuscript.",

year = "1981",

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doi = "10.1016/0003-2697(81)90095-6",

language = "English (US)",

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pages = "407--415",

journal = "Analytical Biochemistry",

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T1 - A method for measurement of free and bound plasma cyst(e)ine

AU - Malloy, Michael H.

AU - Rassin, David K.

AU - Gaull, Gerald E.

N1 - Funding Information: Supported in part by the New York State Office of Mental Retardation and Developmental Disabilities and NIH Grant AM 20236. Dr. Malloy worked in this lab- oratory during the tenure of fellowship under National Research Service Award HD 07000 administered by the Institute of Human Nutrition of Columbia University. The authors are grateful for the expert technical assistance of Ms. Alice Gargano and secretarial assistance of Mrs. Lee Antonucci. We thank Dr. William Heird of Columbia University for helpful discussion during the course of the work as well as Professor Stanford Moore of Rockefeller University and Dr. M. K. Gaitonde of St. George’s Hospital Medical School for their critical readings of the manuscript.

PY - 1981/5/15

Y1 - 1981/5/15

N2 - A method for the measurement of free and bound cyst(e)ine and the sum thereof has been developed. In adult human blood, cyst(e)ine is distributed equally between that which is bound to plasma proteins and that which is free. Cyst(e)ine is bound predominantly to albumin, and this binding is not an in vitro artifact. Cysteine bound to plasma proteins may be displaced by homocysteine which competes for the available sulfhydryl groups of plasma proteins. Rats starved for 8 days had a significant decrease in both plasma free cyst(e)ine and bound cysteine. These data suggest that present methods for the determination of plasma cyst(e)ine under-estimate the quantity of cyst(e)ine in the plasma available for cellular metabolism.

AB - A method for the measurement of free and bound cyst(e)ine and the sum thereof has been developed. In adult human blood, cyst(e)ine is distributed equally between that which is bound to plasma proteins and that which is free. Cyst(e)ine is bound predominantly to albumin, and this binding is not an in vitro artifact. Cysteine bound to plasma proteins may be displaced by homocysteine which competes for the available sulfhydryl groups of plasma proteins. Rats starved for 8 days had a significant decrease in both plasma free cyst(e)ine and bound cysteine. These data suggest that present methods for the determination of plasma cyst(e)ine under-estimate the quantity of cyst(e)ine in the plasma available for cellular metabolism.

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JO - Analytical Biochemistry

JF - Analytical Biochemistry

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A method for measurement of free and bound plasma cyst(e)ine

Abstract

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