A method for producing unbiased histograms of neuronal profile sizes

Carolyn M. Pover, Marshall H. Orr, Richard E. Coggeshall

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

An important goal in neuroscience is to produce frequency distribution curves or histograms that relate numbers of cells to their sizes. Unfortunately such histograms, which are extremely common, are biased. Some of the reasons are inadequate attention to sampling paradigms, the lack of assurance that the section through the center of the cell is measured, and that large cells will have more profiles than small cells. The first goal of this paper is to illustrate these biases for dorsal root ganglion cells by showing significant statistical differences between histograms prepared the classic way and histograms produced by unbiased methods. The differences are particularly dramatic for plastic embedded material. The second goal of the paper is to describe an unbiased method for obtaining these histograms. The procedure is 2-fold. Cells are chosen in an unbiased way (unbiased in this sense means that every cell has an equal chance of being chosen), and then the largest profile of the cell is measured. We further suggest that these histograms be accompanied by analyses of cell volume, since volumes of cells rather than diameters or areas of cell profiles, will be the future measure of choice when considering the sizes of cells (or any other particles of biologic interest).

Original languageEnglish (US)
Pages (from-to)123-131
Number of pages9
JournalJournal of Neuroscience Methods
Volume49
Issue number1-2
DOIs
StatePublished - 1993

Fingerprint

Cell Size
Spinal Ganglia
Neurosciences
Plastics
Cell Count

Keywords

  • Cell size
  • Disector
  • Dorsal root ganglion cell

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

A method for producing unbiased histograms of neuronal profile sizes. / Pover, Carolyn M.; Orr, Marshall H.; Coggeshall, Richard E.

In: Journal of Neuroscience Methods, Vol. 49, No. 1-2, 1993, p. 123-131.

Research output: Contribution to journalArticle

Pover, Carolyn M. ; Orr, Marshall H. ; Coggeshall, Richard E. / A method for producing unbiased histograms of neuronal profile sizes. In: Journal of Neuroscience Methods. 1993 ; Vol. 49, No. 1-2. pp. 123-131.
@article{a2ca5cbf3e60472dad48fbd49b85ddc6,
title = "A method for producing unbiased histograms of neuronal profile sizes",
abstract = "An important goal in neuroscience is to produce frequency distribution curves or histograms that relate numbers of cells to their sizes. Unfortunately such histograms, which are extremely common, are biased. Some of the reasons are inadequate attention to sampling paradigms, the lack of assurance that the section through the center of the cell is measured, and that large cells will have more profiles than small cells. The first goal of this paper is to illustrate these biases for dorsal root ganglion cells by showing significant statistical differences between histograms prepared the classic way and histograms produced by unbiased methods. The differences are particularly dramatic for plastic embedded material. The second goal of the paper is to describe an unbiased method for obtaining these histograms. The procedure is 2-fold. Cells are chosen in an unbiased way (unbiased in this sense means that every cell has an equal chance of being chosen), and then the largest profile of the cell is measured. We further suggest that these histograms be accompanied by analyses of cell volume, since volumes of cells rather than diameters or areas of cell profiles, will be the future measure of choice when considering the sizes of cells (or any other particles of biologic interest).",
keywords = "Cell size, Disector, Dorsal root ganglion cell",
author = "Pover, {Carolyn M.} and Orr, {Marshall H.} and Coggeshall, {Richard E.}",
year = "1993",
doi = "10.1016/0165-0270(93)90116-9",
language = "English (US)",
volume = "49",
pages = "123--131",
journal = "Journal of Neuroscience Methods",
issn = "0165-0270",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - A method for producing unbiased histograms of neuronal profile sizes

AU - Pover, Carolyn M.

AU - Orr, Marshall H.

AU - Coggeshall, Richard E.

PY - 1993

Y1 - 1993

N2 - An important goal in neuroscience is to produce frequency distribution curves or histograms that relate numbers of cells to their sizes. Unfortunately such histograms, which are extremely common, are biased. Some of the reasons are inadequate attention to sampling paradigms, the lack of assurance that the section through the center of the cell is measured, and that large cells will have more profiles than small cells. The first goal of this paper is to illustrate these biases for dorsal root ganglion cells by showing significant statistical differences between histograms prepared the classic way and histograms produced by unbiased methods. The differences are particularly dramatic for plastic embedded material. The second goal of the paper is to describe an unbiased method for obtaining these histograms. The procedure is 2-fold. Cells are chosen in an unbiased way (unbiased in this sense means that every cell has an equal chance of being chosen), and then the largest profile of the cell is measured. We further suggest that these histograms be accompanied by analyses of cell volume, since volumes of cells rather than diameters or areas of cell profiles, will be the future measure of choice when considering the sizes of cells (or any other particles of biologic interest).

AB - An important goal in neuroscience is to produce frequency distribution curves or histograms that relate numbers of cells to their sizes. Unfortunately such histograms, which are extremely common, are biased. Some of the reasons are inadequate attention to sampling paradigms, the lack of assurance that the section through the center of the cell is measured, and that large cells will have more profiles than small cells. The first goal of this paper is to illustrate these biases for dorsal root ganglion cells by showing significant statistical differences between histograms prepared the classic way and histograms produced by unbiased methods. The differences are particularly dramatic for plastic embedded material. The second goal of the paper is to describe an unbiased method for obtaining these histograms. The procedure is 2-fold. Cells are chosen in an unbiased way (unbiased in this sense means that every cell has an equal chance of being chosen), and then the largest profile of the cell is measured. We further suggest that these histograms be accompanied by analyses of cell volume, since volumes of cells rather than diameters or areas of cell profiles, will be the future measure of choice when considering the sizes of cells (or any other particles of biologic interest).

KW - Cell size

KW - Disector

KW - Dorsal root ganglion cell

UR - http://www.scopus.com/inward/record.url?scp=0027224092&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027224092&partnerID=8YFLogxK

U2 - 10.1016/0165-0270(93)90116-9

DO - 10.1016/0165-0270(93)90116-9

M3 - Article

VL - 49

SP - 123

EP - 131

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

SN - 0165-0270

IS - 1-2

ER -