A method for specific diagnosis of Rocky Mountain spotted fever on fixed, paraffin-embedded tissue by immunofluorescence

David Walker, B. G. Cain

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

For specific demonstration of Rickettsia rickettsii in fixed, paraffin-embedded tissues, the technique of trypsin digestion of deparaffinized, rehydrated sections was investigated. It was determined that 3.5 hr of digestion was optimal for achieving discrete, bright, green immunofluorescence of rickettsiae. At autopsy kidneys from seven of 10 cases of probable Rocky Mountain spotted fever contained structures that were specifically stained and that has the size and shape of rickettsiae. These structures were strictly limited to the endothelium and vascular walls of renal capillaries, veins, and arteries. Results from controls indicated the specificity of the immunofluorescence. R. rickettsii may be demonstrated by this method with greater sensitivity and specificity than by current histological methods. The technique allows retrospective analysis of certain organs for pathogenesis of involvement in Rocky Mountain spotted fever and offers a specific diagnostic test.

Original languageEnglish (US)
Pages (from-to)206-209
Number of pages4
JournalJournal of Infectious Diseases
Volume137
Issue number2
StatePublished - 1978
Externally publishedYes

Fingerprint

Rickettsia rickettsii
Rocky Mountain Spotted Fever
Rickettsia
Paraffin
Fluorescent Antibody Technique
Digestion
Renal Veins
Vascular Endothelium
Renal Artery
Routine Diagnostic Tests
Trypsin
Autopsy
Kidney
Sensitivity and Specificity

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

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abstract = "For specific demonstration of Rickettsia rickettsii in fixed, paraffin-embedded tissues, the technique of trypsin digestion of deparaffinized, rehydrated sections was investigated. It was determined that 3.5 hr of digestion was optimal for achieving discrete, bright, green immunofluorescence of rickettsiae. At autopsy kidneys from seven of 10 cases of probable Rocky Mountain spotted fever contained structures that were specifically stained and that has the size and shape of rickettsiae. These structures were strictly limited to the endothelium and vascular walls of renal capillaries, veins, and arteries. Results from controls indicated the specificity of the immunofluorescence. R. rickettsii may be demonstrated by this method with greater sensitivity and specificity than by current histological methods. The technique allows retrospective analysis of certain organs for pathogenesis of involvement in Rocky Mountain spotted fever and offers a specific diagnostic test.",
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