A method for the isolation and identification of dengue viruses, using mosquito cell cultures

R. B. Tesh

Research output: Contribution to journalArticle

135 Scopus citations

Abstract

An improved method for the isolation and identification of dengue viruses is described. Viruses were isolated in mosquito cell cultures (C6/36 or AP-61), identified by indirect fluorescent antibody technique, and typed by complement-fixation test, using the cell culture fluid as antigen. The sensitivity of this method was compared with mosquito inoculation in comparative titrations of 16 low passage dengue virus strains. Although lower virus titers were obtained by the mosquito cell culture technique, its decreased sensitivity was compensated for by the much larger volume (588x) which could be assayed. By incubating the mosquito cells at 32°C, dengue viruses can be identified and typed within 6 days after inoculation.

Original languageEnglish (US)
Pages (from-to)1053-1059
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume28
Issue number6
DOIs
StatePublished - Jan 1 1979

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

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