A model utilizing adult murine stem cells for creation of personalized islets for transplantation

J. Wang, L. J. Song, D. A. Gerber, Jeffrey Fair, L. Rice, M. Lapaglia, K. A. Andreoni

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Clinical islet cell transplantation has demonstrated great promise for diabetes treatment. Two major obstacles are the organ donor shortage and the immunoresponse. The purpose of this study was to create a model using the patient's own adult stem cell sources, possibly in combination with non-self cells, such as pancreatic, hepatic, or embryonic stem cells, to create "personalized" islets. We hypothesize that the reconstructed islets have the normal capability to produce insulin and glucagon with reduced immunoresponses after transplantation. Stem cells are a proliferating population of master cells that have the ability for self-renewal and multilineage differentiation. The recently developed photolithograph-based, biologic, microelectromechanic system (BioMEMS) technique supplies a useful tool for biomedical applications. Our lab has developed a novel method that integrates the adult stem cell and BioMEMS to reconstruct personalized islets. We selected islet-derived progenitor cells (IPC) for repairing and reconstructing STZ-diabetic islets. A6(+)/PYY(+) or A6(+)/ngn3(+) cells were selected to manipulate the neoislets. After 3 to 4 weeks in culture, the reconstructed cells formed islet-like clusters containing insulin or glucagon producing cells. The pilot results showed the ability of these reconstructed islets to correct hyperglycemia when transplanted into a STZ-diabetic isograft mouse model. Although several technical problems remain with the mouse model, namely, the difficulty to collect enough islets from a single mouse because of animal size, the mouse isograft model is suitable for personalized islet development.

Original languageEnglish (US)
Pages (from-to)1188-1190
Number of pages3
JournalTransplantation Proceedings
Volume36
Issue number4
DOIs
StatePublished - May 2004
Externally publishedYes

Fingerprint

Islets of Langerhans Transplantation
Adult Stem Cells
Isografts
Glucagon
Islets of Langerhans
Stem Cells
Insulin
Cell Transplantation
Embryonic Stem Cells
Hyperglycemia
Cell Culture Techniques
Transplantation
Tissue Donors
Liver
Population
Therapeutics

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Cite this

A model utilizing adult murine stem cells for creation of personalized islets for transplantation. / Wang, J.; Song, L. J.; Gerber, D. A.; Fair, Jeffrey; Rice, L.; Lapaglia, M.; Andreoni, K. A.

In: Transplantation Proceedings, Vol. 36, No. 4, 05.2004, p. 1188-1190.

Research output: Contribution to journalArticle

Wang, J. ; Song, L. J. ; Gerber, D. A. ; Fair, Jeffrey ; Rice, L. ; Lapaglia, M. ; Andreoni, K. A. / A model utilizing adult murine stem cells for creation of personalized islets for transplantation. In: Transplantation Proceedings. 2004 ; Vol. 36, No. 4. pp. 1188-1190.
@article{13018bc0455a4fbc965ad53a46eeca49,
title = "A model utilizing adult murine stem cells for creation of personalized islets for transplantation",
abstract = "Clinical islet cell transplantation has demonstrated great promise for diabetes treatment. Two major obstacles are the organ donor shortage and the immunoresponse. The purpose of this study was to create a model using the patient's own adult stem cell sources, possibly in combination with non-self cells, such as pancreatic, hepatic, or embryonic stem cells, to create {"}personalized{"} islets. We hypothesize that the reconstructed islets have the normal capability to produce insulin and glucagon with reduced immunoresponses after transplantation. Stem cells are a proliferating population of master cells that have the ability for self-renewal and multilineage differentiation. The recently developed photolithograph-based, biologic, microelectromechanic system (BioMEMS) technique supplies a useful tool for biomedical applications. Our lab has developed a novel method that integrates the adult stem cell and BioMEMS to reconstruct personalized islets. We selected islet-derived progenitor cells (IPC) for repairing and reconstructing STZ-diabetic islets. A6(+)/PYY(+) or A6(+)/ngn3(+) cells were selected to manipulate the neoislets. After 3 to 4 weeks in culture, the reconstructed cells formed islet-like clusters containing insulin or glucagon producing cells. The pilot results showed the ability of these reconstructed islets to correct hyperglycemia when transplanted into a STZ-diabetic isograft mouse model. Although several technical problems remain with the mouse model, namely, the difficulty to collect enough islets from a single mouse because of animal size, the mouse isograft model is suitable for personalized islet development.",
author = "J. Wang and Song, {L. J.} and Gerber, {D. A.} and Jeffrey Fair and L. Rice and M. Lapaglia and Andreoni, {K. A.}",
year = "2004",
month = "5",
doi = "10.1016/j.transproceed.2004.05.025",
language = "English (US)",
volume = "36",
pages = "1188--1190",
journal = "Transplantation Proceedings",
issn = "0041-1345",
publisher = "Elsevier USA",
number = "4",

}

TY - JOUR

T1 - A model utilizing adult murine stem cells for creation of personalized islets for transplantation

AU - Wang, J.

AU - Song, L. J.

AU - Gerber, D. A.

AU - Fair, Jeffrey

AU - Rice, L.

AU - Lapaglia, M.

AU - Andreoni, K. A.

PY - 2004/5

Y1 - 2004/5

N2 - Clinical islet cell transplantation has demonstrated great promise for diabetes treatment. Two major obstacles are the organ donor shortage and the immunoresponse. The purpose of this study was to create a model using the patient's own adult stem cell sources, possibly in combination with non-self cells, such as pancreatic, hepatic, or embryonic stem cells, to create "personalized" islets. We hypothesize that the reconstructed islets have the normal capability to produce insulin and glucagon with reduced immunoresponses after transplantation. Stem cells are a proliferating population of master cells that have the ability for self-renewal and multilineage differentiation. The recently developed photolithograph-based, biologic, microelectromechanic system (BioMEMS) technique supplies a useful tool for biomedical applications. Our lab has developed a novel method that integrates the adult stem cell and BioMEMS to reconstruct personalized islets. We selected islet-derived progenitor cells (IPC) for repairing and reconstructing STZ-diabetic islets. A6(+)/PYY(+) or A6(+)/ngn3(+) cells were selected to manipulate the neoislets. After 3 to 4 weeks in culture, the reconstructed cells formed islet-like clusters containing insulin or glucagon producing cells. The pilot results showed the ability of these reconstructed islets to correct hyperglycemia when transplanted into a STZ-diabetic isograft mouse model. Although several technical problems remain with the mouse model, namely, the difficulty to collect enough islets from a single mouse because of animal size, the mouse isograft model is suitable for personalized islet development.

AB - Clinical islet cell transplantation has demonstrated great promise for diabetes treatment. Two major obstacles are the organ donor shortage and the immunoresponse. The purpose of this study was to create a model using the patient's own adult stem cell sources, possibly in combination with non-self cells, such as pancreatic, hepatic, or embryonic stem cells, to create "personalized" islets. We hypothesize that the reconstructed islets have the normal capability to produce insulin and glucagon with reduced immunoresponses after transplantation. Stem cells are a proliferating population of master cells that have the ability for self-renewal and multilineage differentiation. The recently developed photolithograph-based, biologic, microelectromechanic system (BioMEMS) technique supplies a useful tool for biomedical applications. Our lab has developed a novel method that integrates the adult stem cell and BioMEMS to reconstruct personalized islets. We selected islet-derived progenitor cells (IPC) for repairing and reconstructing STZ-diabetic islets. A6(+)/PYY(+) or A6(+)/ngn3(+) cells were selected to manipulate the neoislets. After 3 to 4 weeks in culture, the reconstructed cells formed islet-like clusters containing insulin or glucagon producing cells. The pilot results showed the ability of these reconstructed islets to correct hyperglycemia when transplanted into a STZ-diabetic isograft mouse model. Although several technical problems remain with the mouse model, namely, the difficulty to collect enough islets from a single mouse because of animal size, the mouse isograft model is suitable for personalized islet development.

UR - http://www.scopus.com/inward/record.url?scp=2942670710&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942670710&partnerID=8YFLogxK

U2 - 10.1016/j.transproceed.2004.05.025

DO - 10.1016/j.transproceed.2004.05.025

M3 - Article

VL - 36

SP - 1188

EP - 1190

JO - Transplantation Proceedings

JF - Transplantation Proceedings

SN - 0041-1345

IS - 4

ER -