A modified rapid method of nucleic acid isolation from suspension of matured virus: Applied in restriction analysis of DNA from an adenovirus prototype strain and a patient isolate

C. T. Le, G. C. Gray, S. K. Poddar

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

This report describes a method for the isolation of nucleic acid from a suspension of matured virus. Nucleic acid (DNA) was isolated from a prototype strain of adenovirus type 7 and a clinical isolate of adenovirus type 7. Instead of the usual method of ultracentifugation, a filtration method was applied to concentrate the virus rapidly and nucleic acid was then isolated by a standard phenol/chloroform/isoamyl-alcohol extraction procedure. The DNA was found to be sufficiently purified to generate a reproducible restriction endonuclease digestion pattern. The clinical isolate of adenovirus type 7 revealed loss of restriction site for the endonuclease HindIII when compared with the prototype strain.

Original languageEnglish (US)
Pages (from-to)571-574
Number of pages4
JournalJournal of Medical Microbiology
Volume50
Issue number6
DOIs
StatePublished - 2001
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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