A mouse model for evaluation of prophylaxis and therapy of ebola hemorrhagic fever

Mike Bray, Kelly Davis, Thomas Geisbert, Connie Schmaljohn, John Huggins

Research output: Contribution to journalArticle

289 Citations (Scopus)

Abstract

The Zaire subtype of Ebola virus (EBO-Z) is lethal for newborn mice, but adult mice are resistant to the virus, which prevents their use as an animal model of lethal Ebola infection. We serially passed EBO-Z virus in progressively older suckling mice, eventually obtaining a plaque-purified virus that was lethal for mature, immunocompetent BALB/c and C57BL/6 inbred and ICR (CD-1) outbred mice. Pathologic changes in the liver and spleen of infected mice resembled those in EBO-Z-infected primates. Virus titers in these tissues reached 109 pfu/g. The LD50 of mouse-adapted EBO-Z virus inoculated into the peritoneal cavity was ~1 virion. Mice were resistant to large doses of the same virus inoculated subcutaneously, intradermally, or intramuscularly. Mice injected peripherally with mouse-adapted or intraperitoneally with non-adapted EBO-Z virus resisted subsequent challenge with mouse-adapted virus.

Original languageEnglish (US)
Pages (from-to)651-661
Number of pages11
JournalJournal of Infectious Diseases
Volume178
Issue number3
StatePublished - 1998
Externally publishedYes

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Ebola Hemorrhagic Fever
Viruses
Therapeutics
Ebolavirus
Lethal Dose 50
Peritoneal Cavity
Viral Load
Virion
Primates

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

A mouse model for evaluation of prophylaxis and therapy of ebola hemorrhagic fever. / Bray, Mike; Davis, Kelly; Geisbert, Thomas; Schmaljohn, Connie; Huggins, John.

In: Journal of Infectious Diseases, Vol. 178, No. 3, 1998, p. 651-661.

Research output: Contribution to journalArticle

Bray, M, Davis, K, Geisbert, T, Schmaljohn, C & Huggins, J 1998, 'A mouse model for evaluation of prophylaxis and therapy of ebola hemorrhagic fever', Journal of Infectious Diseases, vol. 178, no. 3, pp. 651-661.
Bray, Mike ; Davis, Kelly ; Geisbert, Thomas ; Schmaljohn, Connie ; Huggins, John. / A mouse model for evaluation of prophylaxis and therapy of ebola hemorrhagic fever. In: Journal of Infectious Diseases. 1998 ; Vol. 178, No. 3. pp. 651-661.
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