A mouse model of Leishmania braziliensis braziliensis infection produced by coinjection with sand fly saliva

John Samuelson, Ethan Lerner, Robert Tesh, Richard Titus

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

To development a reliable murine model of Leishmania braziliensis braziliensis infection, parasites were injected into BALB/c mice in the presence of phlebotomine sand fly salivary gland lysates, which have previously been shown to greatly increase the infectivity of L. major in mice. When injected with salivary gland lysates, L. braziliensis braziliensis produced progressively enlarging cutaneous nodules, containing many macrophages filled with Leishmania amastigotes. In contrast, L. braziliensis injected without gland extracts produced small and rapidly regressing lesions. Isoenzyme analysis, monoclonal antibodies, and the polymerase chain reaction with L. braziliensis-specific oligonucleotide primers and probes confirmed that parasites causing the lesions were L. braziliensis.

Original languageEnglish (US)
Pages (from-to)49-54
Number of pages6
JournalJournal of Experimental Medicine
Volume173
Issue number1
StatePublished - 1991
Externally publishedYes

Fingerprint

Leishmania braziliensis
Psychodidae
Saliva
Infection
Salivary Glands
Parasitic Diseases
DNA Primers
Oligonucleotide Probes
Leishmania
Isoenzymes
Parasites
Macrophages
Monoclonal Antibodies
Polymerase Chain Reaction
Skin

ASJC Scopus subject areas

  • Immunology

Cite this

A mouse model of Leishmania braziliensis braziliensis infection produced by coinjection with sand fly saliva. / Samuelson, John; Lerner, Ethan; Tesh, Robert; Titus, Richard.

In: Journal of Experimental Medicine, Vol. 173, No. 1, 1991, p. 49-54.

Research output: Contribution to journalArticle

Samuelson, John ; Lerner, Ethan ; Tesh, Robert ; Titus, Richard. / A mouse model of Leishmania braziliensis braziliensis infection produced by coinjection with sand fly saliva. In: Journal of Experimental Medicine. 1991 ; Vol. 173, No. 1. pp. 49-54.
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