A mouse model of Leishmania braziliensis braziliensis infection produced by coinjection with sand fly saliva

J. Samuelson, E. Lerner, R. Tesh, R. Titus

Research output: Contribution to journalArticle

101 Scopus citations

Abstract

To development a reliable murine model of Leishmania braziliensis braziliensis infection, parasites were injected into BALB/c mice in the presence of phlebotomine sand fly salivary gland lysates, which have previously been shown to greatly increase the infectivity of L. major in mice. When injected with salivary gland lysates, L. braziliensis braziliensis produced progressively enlarging cutaneous nodules, containing many macrophages filled with Leishmania amastigotes. In contrast, L. braziliensis injected without gland extracts produced small and rapidly regressing lesions. Isoenzyme analysis, monoclonal antibodies, and the polymerase chain reaction with L. braziliensis-specific oligonucleotide primers and probes confirmed that parasites causing the lesions were L. braziliensis.

Original languageEnglish (US)
Pages (from-to)49-54
Number of pages6
JournalJournal of Experimental Medicine
Volume173
Issue number1
DOIs
StatePublished - Jan 25 1991
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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