A multi-omic systems approach to elucidating Yersinia virulence mechanisms

Charles Ansong, Alexandra C. Schrimpe-Rutledge, Hugh D. Mitchell, Sadhana Chauhan, Marcus B. Jones, Young Mo Kim, Kathleen McAteer, Brooke L. Deatherage Kaiser, Jennifer L. Dubois, Heather M. Brewer, Bryan C. Frank, Jason E. McDermott, Thomas O. Metz, Scott N. Peterson, Richard D. Smith, Vladimir Motin, Joshua N. Adkins

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The underlying mechanisms that lead to dramatic differences between closely related pathogens are not always readily apparent. For example, the genomes of Yersinia pestis (YP) the causative agent of plague with a high mortality rate and Yersinia pseudotuberculosis (YPT) an enteric pathogen with a modest mortality rate are highly similar with some species specific differences; however the molecular causes of their distinct clinical outcomes remain poorly understood. In this study, a temporal multi-omic analysis of YP and YPT at physiologically relevant temperatures was performed to gain insights into how an acute and highly lethal bacterial pathogen, YP, differs from its less virulent progenitor, YPT. This analysis revealed higher gene and protein expression levels of conserved major virulence factors in YP relative to YPT, including the Yop virulon and the pH6 antigen. This suggests that adaptation in the regulatory architecture, in addition to the presence of unique genetic material, may contribute to the increased pathogenecity of YP relative to YPT. Additionally, global transcriptome and proteome responses of YP and YPT revealed conserved post-transcriptional control of metabolism and the translational machinery including the modulation of glutamate levels in Yersiniae. Finally, the omics data was coupled with a computational network analysis, allowing an efficient prediction of novel Yersinia virulence factors based on gene and protein expression patterns.

Original languageEnglish (US)
Pages (from-to)44-54
Number of pages11
JournalMolecular BioSystems
Volume9
Issue number1
DOIs
StatePublished - 2013

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Yersinia pseudotuberculosis
Yersinia pestis
Yersinia
Systems Analysis
Virulence
Virulence Factors
Gene Expression
Plague
Mortality
Proteome
Transcriptome
Glutamic Acid
Proteins
Genome
Antigens
Temperature
Genes

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

Cite this

Ansong, C., Schrimpe-Rutledge, A. C., Mitchell, H. D., Chauhan, S., Jones, M. B., Kim, Y. M., ... Adkins, J. N. (2013). A multi-omic systems approach to elucidating Yersinia virulence mechanisms. Molecular BioSystems, 9(1), 44-54. https://doi.org/10.1039/c2mb25287b

A multi-omic systems approach to elucidating Yersinia virulence mechanisms. / Ansong, Charles; Schrimpe-Rutledge, Alexandra C.; Mitchell, Hugh D.; Chauhan, Sadhana; Jones, Marcus B.; Kim, Young Mo; McAteer, Kathleen; Deatherage Kaiser, Brooke L.; Dubois, Jennifer L.; Brewer, Heather M.; Frank, Bryan C.; McDermott, Jason E.; Metz, Thomas O.; Peterson, Scott N.; Smith, Richard D.; Motin, Vladimir; Adkins, Joshua N.

In: Molecular BioSystems, Vol. 9, No. 1, 2013, p. 44-54.

Research output: Contribution to journalArticle

Ansong, C, Schrimpe-Rutledge, AC, Mitchell, HD, Chauhan, S, Jones, MB, Kim, YM, McAteer, K, Deatherage Kaiser, BL, Dubois, JL, Brewer, HM, Frank, BC, McDermott, JE, Metz, TO, Peterson, SN, Smith, RD, Motin, V & Adkins, JN 2013, 'A multi-omic systems approach to elucidating Yersinia virulence mechanisms', Molecular BioSystems, vol. 9, no. 1, pp. 44-54. https://doi.org/10.1039/c2mb25287b
Ansong C, Schrimpe-Rutledge AC, Mitchell HD, Chauhan S, Jones MB, Kim YM et al. A multi-omic systems approach to elucidating Yersinia virulence mechanisms. Molecular BioSystems. 2013;9(1):44-54. https://doi.org/10.1039/c2mb25287b
Ansong, Charles ; Schrimpe-Rutledge, Alexandra C. ; Mitchell, Hugh D. ; Chauhan, Sadhana ; Jones, Marcus B. ; Kim, Young Mo ; McAteer, Kathleen ; Deatherage Kaiser, Brooke L. ; Dubois, Jennifer L. ; Brewer, Heather M. ; Frank, Bryan C. ; McDermott, Jason E. ; Metz, Thomas O. ; Peterson, Scott N. ; Smith, Richard D. ; Motin, Vladimir ; Adkins, Joshua N. / A multi-omic systems approach to elucidating Yersinia virulence mechanisms. In: Molecular BioSystems. 2013 ; Vol. 9, No. 1. pp. 44-54.
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