A Multicomponent Animal Virus Isolated from Mosquitoes

Jason T. Ladner, Michael R. Wiley, Brett Beitzel, Albert J. Auguste, Alan P. Dupuis, Michael E. Lindquist, Samuel D. Sibley, Krishna P. Kota, David Fetterer, Gillian Eastwood, David Kimmel, Karla Prieto, Hilda Guzman, Matthew T. Aliota, Daniel Reyes, Ernst E. Brueggemann, Lena St. John, David Hyeroba, Michael Lauck, Thomas C. Friedrich & 15 others David H. O'Connor, Marie C. Gestole, Lisa H. Cazares, Vsevolod Popov, Fanny Castro-Llanos, Tadeusz J. Kochel, Tara Kenny, Bailey White, Michael D. Ward, Jose R. Loaiza, Tony L. Goldberg, Scott Weaver, Laura D. Kramer, Robert B. Tesh, Gustavo Palacios

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

RNA viruses exhibit a variety of genome organization strategies, including multicomponent genomes in which each segment is packaged separately. Although multicomponent genomes are common among viruses infecting plants and fungi, their prevalence among those infecting animals remains unclear. We characterize a multicomponent RNA virus isolated from mosquitoes, designated Guaico Culex virus (GCXV). GCXV belongs to a diverse clade of segmented viruses (Jingmenvirus) related to the prototypically unsegmented Flaviviridae. The GCXV genome comprises five segments, each of which appears to be separately packaged. The smallest segment is not required for replication, and its presence is variable in natural infections. We also describe a variant of Jingmen tick virus, another Jingmenvirus, sequenced from a Ugandan red colobus monkey, thus expanding the host range of this segmented and likely multicomponent virus group. Collectively, this study provides evidence for the existence of multicomponent animal viruses and their potential relevance for animal and human health.

Original languageEnglish (US)
Pages (from-to)357-367
Number of pages11
JournalCell Host and Microbe
Volume20
Issue number3
DOIs
StatePublished - Sep 14 2016

Fingerprint

Culicidae
Viruses
Culex
Genome
RNA Viruses
Colobus
Erythrocebus patas
Flaviviridae
Plant Viruses
Host Specificity
Ticks
Fungi
Health
Infection

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology and Microbiology(all)
  • Cancer Research

Cite this

Ladner, J. T., Wiley, M. R., Beitzel, B., Auguste, A. J., Dupuis, A. P., Lindquist, M. E., ... Palacios, G. (2016). A Multicomponent Animal Virus Isolated from Mosquitoes. Cell Host and Microbe, 20(3), 357-367. https://doi.org/10.1016/j.chom.2016.07.011

A Multicomponent Animal Virus Isolated from Mosquitoes. / Ladner, Jason T.; Wiley, Michael R.; Beitzel, Brett; Auguste, Albert J.; Dupuis, Alan P.; Lindquist, Michael E.; Sibley, Samuel D.; Kota, Krishna P.; Fetterer, David; Eastwood, Gillian; Kimmel, David; Prieto, Karla; Guzman, Hilda; Aliota, Matthew T.; Reyes, Daniel; Brueggemann, Ernst E.; St. John, Lena; Hyeroba, David; Lauck, Michael; Friedrich, Thomas C.; O'Connor, David H.; Gestole, Marie C.; Cazares, Lisa H.; Popov, Vsevolod; Castro-Llanos, Fanny; Kochel, Tadeusz J.; Kenny, Tara; White, Bailey; Ward, Michael D.; Loaiza, Jose R.; Goldberg, Tony L.; Weaver, Scott; Kramer, Laura D.; Tesh, Robert B.; Palacios, Gustavo.

In: Cell Host and Microbe, Vol. 20, No. 3, 14.09.2016, p. 357-367.

Research output: Contribution to journalArticle

Ladner, JT, Wiley, MR, Beitzel, B, Auguste, AJ, Dupuis, AP, Lindquist, ME, Sibley, SD, Kota, KP, Fetterer, D, Eastwood, G, Kimmel, D, Prieto, K, Guzman, H, Aliota, MT, Reyes, D, Brueggemann, EE, St. John, L, Hyeroba, D, Lauck, M, Friedrich, TC, O'Connor, DH, Gestole, MC, Cazares, LH, Popov, V, Castro-Llanos, F, Kochel, TJ, Kenny, T, White, B, Ward, MD, Loaiza, JR, Goldberg, TL, Weaver, S, Kramer, LD, Tesh, RB & Palacios, G 2016, 'A Multicomponent Animal Virus Isolated from Mosquitoes', Cell Host and Microbe, vol. 20, no. 3, pp. 357-367. https://doi.org/10.1016/j.chom.2016.07.011
Ladner JT, Wiley MR, Beitzel B, Auguste AJ, Dupuis AP, Lindquist ME et al. A Multicomponent Animal Virus Isolated from Mosquitoes. Cell Host and Microbe. 2016 Sep 14;20(3):357-367. https://doi.org/10.1016/j.chom.2016.07.011
Ladner, Jason T. ; Wiley, Michael R. ; Beitzel, Brett ; Auguste, Albert J. ; Dupuis, Alan P. ; Lindquist, Michael E. ; Sibley, Samuel D. ; Kota, Krishna P. ; Fetterer, David ; Eastwood, Gillian ; Kimmel, David ; Prieto, Karla ; Guzman, Hilda ; Aliota, Matthew T. ; Reyes, Daniel ; Brueggemann, Ernst E. ; St. John, Lena ; Hyeroba, David ; Lauck, Michael ; Friedrich, Thomas C. ; O'Connor, David H. ; Gestole, Marie C. ; Cazares, Lisa H. ; Popov, Vsevolod ; Castro-Llanos, Fanny ; Kochel, Tadeusz J. ; Kenny, Tara ; White, Bailey ; Ward, Michael D. ; Loaiza, Jose R. ; Goldberg, Tony L. ; Weaver, Scott ; Kramer, Laura D. ; Tesh, Robert B. ; Palacios, Gustavo. / A Multicomponent Animal Virus Isolated from Mosquitoes. In: Cell Host and Microbe. 2016 ; Vol. 20, No. 3. pp. 357-367.
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