A Multiplex Microsphere Immunoassay for Zika Virus Diagnosis

Susan J. Wong, Andrea Furuya, Jing Zou, Xuping Xie, Alan P. Dupuis, Laura D. Kramer, Pei-Yong Shi

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Rapid and accurate diagnosis of infectious agents is essential for patient care, disease control, and countermeasure development. The present serologic diagnosis of Zika virus (ZIKV) infection relies mainly on IgM-capture ELISA which is confounded with the flaw of cross-reactivity among different flaviviruses. In this communication, we report a multiplex microsphere immunoassay (MIA) that captures the diagnostic power of viral envelope protein (that elicits robust, yet cross-reactive antibodies to other flaviviruses) and the differential power of viral nonstructural proteins NS1 and NS5 (that induce more virus-type specific antibodies). Using 153 patient specimens with known ZIKV and/or dengue virus (DENV; a closely related flavivirus) infections, we showed that (i) ZIKV envelope-based MIA is equivalent or more sensitive than IgM-capture ELISA in diagnosing ZIKV infection, (ii) antibody responses to NS1 and NS5 proteins are more ZIKV-specific than antibody response to envelope protein, (iii) inclusion of NS1 and NS5 in the MIA improves the diagnostic accuracy when compared with the MIA that uses envelope protein alone. The multiplex MIA achieves a rapid diagnosis (turnaround time. <. 4. h) and requires small specimen volume (10. μl) in a single reaction. This serologic assay could be developed for use in clinical diagnosis of ZIKV infection and for monitoring immune responses in vaccine trials.

Original languageEnglish (US)
JournalEBioMedicine
DOIs
StateAccepted/In press - Nov 29 2016

Fingerprint

Microspheres
Viruses
Immunoassay
Flavivirus
Antibody Formation
Immunoglobulin M
Flavivirus Infections
Viral Nonstructural Proteins
Enzyme-Linked Immunosorbent Assay
Antibodies
Viral Envelope Proteins
Immunologic Monitoring
Dengue Virus
Proteins
Disease control
Patient Care
Vaccines
Turnaround time
Zika Virus
Assays

Keywords

  • Diagnosis
  • Multiplex microsphere immunoassay
  • Serologic assay
  • Zika virus

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Wong, S. J., Furuya, A., Zou, J., Xie, X., Dupuis, A. P., Kramer, L. D., & Shi, P-Y. (Accepted/In press). A Multiplex Microsphere Immunoassay for Zika Virus Diagnosis. EBioMedicine. https://doi.org/10.1016/j.ebiom.2017.01.008

A Multiplex Microsphere Immunoassay for Zika Virus Diagnosis. / Wong, Susan J.; Furuya, Andrea; Zou, Jing; Xie, Xuping; Dupuis, Alan P.; Kramer, Laura D.; Shi, Pei-Yong.

In: EBioMedicine, 29.11.2016.

Research output: Contribution to journalArticle

Wong SJ, Furuya A, Zou J, Xie X, Dupuis AP, Kramer LD et al. A Multiplex Microsphere Immunoassay for Zika Virus Diagnosis. EBioMedicine. 2016 Nov 29. https://doi.org/10.1016/j.ebiom.2017.01.008
Wong, Susan J. ; Furuya, Andrea ; Zou, Jing ; Xie, Xuping ; Dupuis, Alan P. ; Kramer, Laura D. ; Shi, Pei-Yong. / A Multiplex Microsphere Immunoassay for Zika Virus Diagnosis. In: EBioMedicine. 2016.
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