A multiplex PCR procedure for polymorphic analysis of GSTM1 and GSTT1 genes in population studies

Sherif Z. Abdel-Rahman, Randa A. El-Zein, Wagida A. Anwar, William W. Au

Research output: Contribution to journalArticle

222 Scopus citations

Abstract

A deletion polymorphism in glutathione S-transferase theta (GSTT1) gene was recently discovered in humans. Similar to the GSTM1 gene, GSTT1 is also recognized as a risk modifier in exposed populations. To evaluate the role of genetic polymorphism in health effects, the combined genetic polymorphism of different genes should be taken into consideration. In the present study, we have developed a multiplex PCR approach for simultaneous replication of both genes for molecular analysis. The multiplex PCR protocol was validated using donor DNA with different polymorphic combinations for both genes from two different ethnic populations (North Americans and Egyptians). The prevalence of the GSTM1 null genotype was 51% among North Americans and 44% among Egyptians. The prevalence of the GSTT1 null genotype was 15% among North Americans and 14.7% among Egyptians. Combined polymorphism analysis of both genes revealed that 6.3% of North Americans harbor the deleted genotype of both genes compared to 8.8% of the Egyptians. The data indicate that there is no major difference in allelic distribution of both genes between the ethnic populations. The multiplex PCR assay used in this study has the advantage of reducing the time, effort and cost required to carry out such analysis. It will also significantly enhance the ability to use genetic screening techniques as a potential tool for early detection of health outcomes in exposed populations.

Original languageEnglish (US)
Pages (from-to)229-233
Number of pages5
JournalCancer Letters
Volume107
Issue number2
DOIs
StatePublished - Oct 22 1996

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Keywords

  • Egyptians
  • Electrophoresis
  • GSTM1
  • GSTT1
  • Genetic polymorphism
  • Multiplex
  • North Americans
  • PCR
  • Population

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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