Abstract
A deletion polymorphism in glutathione S-transferase theta (GSTT1) gene was recently discovered in humans. Similar to the GSTM1 gene, GSTT1 is also recognized as a risk modifier in exposed populations. To evaluate the role of genetic polymorphism in health effects, the combined genetic polymorphism of different genes should be taken into consideration. In the present study, we have developed a multiplex PCR approach for simultaneous replication of both genes for molecular analysis. The multiplex PCR protocol was validated using donor DNA with different polymorphic combinations for both genes from two different ethnic populations (North Americans and Egyptians). The prevalence of the GSTM1 null genotype was 51% among North Americans and 44% among Egyptians. The prevalence of the GSTT1 null genotype was 15% among North Americans and 14.7% among Egyptians. Combined polymorphism analysis of both genes revealed that 6.3% of North Americans harbor the deleted genotype of both genes compared to 8.8% of the Egyptians. The data indicate that there is no major difference in allelic distribution of both genes between the ethnic populations. The multiplex PCR assay used in this study has the advantage of reducing the time, effort and cost required to carry out such analysis. It will also significantly enhance the ability to use genetic screening techniques as a potential tool for early detection of health outcomes in exposed populations.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 229-233 |
| Number of pages | 5 |
| Journal | Cancer Letters |
| Volume | 107 |
| Issue number | 2 |
| DOIs | |
| State | Published - Oct 22 1996 |
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Keywords
- Egyptians
- Electrophoresis
- Genetic polymorphism
- GSTM1
- GSTT1
- Multiplex
- North Americans
- PCR
- Population
ASJC Scopus subject areas
- Cancer Research
- Molecular Biology
- Oncology
Cite this
A multiplex PCR procedure for polymorphic analysis of GSTM1 and GSTT1 genes in population studies. / Abdel-Rahman, Sherif; El-Zein, Randa A.; Anwar, Wagida A.; Au, William W.
In: Cancer Letters, Vol. 107, No. 2, 22.10.1996, p. 229-233.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A multiplex PCR procedure for polymorphic analysis of GSTM1 and GSTT1 genes in population studies
AU - Abdel-Rahman, Sherif
AU - El-Zein, Randa A.
AU - Anwar, Wagida A.
AU - Au, William W.
PY - 1996/10/22
Y1 - 1996/10/22
N2 - A deletion polymorphism in glutathione S-transferase theta (GSTT1) gene was recently discovered in humans. Similar to the GSTM1 gene, GSTT1 is also recognized as a risk modifier in exposed populations. To evaluate the role of genetic polymorphism in health effects, the combined genetic polymorphism of different genes should be taken into consideration. In the present study, we have developed a multiplex PCR approach for simultaneous replication of both genes for molecular analysis. The multiplex PCR protocol was validated using donor DNA with different polymorphic combinations for both genes from two different ethnic populations (North Americans and Egyptians). The prevalence of the GSTM1 null genotype was 51% among North Americans and 44% among Egyptians. The prevalence of the GSTT1 null genotype was 15% among North Americans and 14.7% among Egyptians. Combined polymorphism analysis of both genes revealed that 6.3% of North Americans harbor the deleted genotype of both genes compared to 8.8% of the Egyptians. The data indicate that there is no major difference in allelic distribution of both genes between the ethnic populations. The multiplex PCR assay used in this study has the advantage of reducing the time, effort and cost required to carry out such analysis. It will also significantly enhance the ability to use genetic screening techniques as a potential tool for early detection of health outcomes in exposed populations.
AB - A deletion polymorphism in glutathione S-transferase theta (GSTT1) gene was recently discovered in humans. Similar to the GSTM1 gene, GSTT1 is also recognized as a risk modifier in exposed populations. To evaluate the role of genetic polymorphism in health effects, the combined genetic polymorphism of different genes should be taken into consideration. In the present study, we have developed a multiplex PCR approach for simultaneous replication of both genes for molecular analysis. The multiplex PCR protocol was validated using donor DNA with different polymorphic combinations for both genes from two different ethnic populations (North Americans and Egyptians). The prevalence of the GSTM1 null genotype was 51% among North Americans and 44% among Egyptians. The prevalence of the GSTT1 null genotype was 15% among North Americans and 14.7% among Egyptians. Combined polymorphism analysis of both genes revealed that 6.3% of North Americans harbor the deleted genotype of both genes compared to 8.8% of the Egyptians. The data indicate that there is no major difference in allelic distribution of both genes between the ethnic populations. The multiplex PCR assay used in this study has the advantage of reducing the time, effort and cost required to carry out such analysis. It will also significantly enhance the ability to use genetic screening techniques as a potential tool for early detection of health outcomes in exposed populations.
KW - Egyptians
KW - Electrophoresis
KW - Genetic polymorphism
KW - GSTM1
KW - GSTT1
KW - Multiplex
KW - North Americans
KW - PCR
KW - Population
UR - http://www.scopus.com/inward/record.url?scp=0030598162&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030598162&partnerID=8YFLogxK
U2 - 10.1016/0304-3835(96)04832-X
DO - 10.1016/0304-3835(96)04832-X
M3 - Article
C2 - 8947518
AN - SCOPUS:0030598162
VL - 107
SP - 229
EP - 233
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 2
ER -