A multivalent adsorption apparatus explains the broad host range of phage phi92

A comprehensive genomic and structural analysis

David Schwarzer, Falk F R Buettner, Christopher Browning, Sergey Nazarov, Wolfgang Rabsch, Andrea Bethe, Astrid Oberbeck, Valorie D. Bowman, Katharina Stummeyer, Martina Mühlenhoff, Petr Leiman, Rita Gerardy-Schahn

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Bacteriophage phi92 is a large, lytic myovirus isolated in 1983 from pathogenic Escherichia coli strains that carry a polysialic acid capsule. Here we report the genome organization of phi92, the cryoelectron microscopy reconstruction of its virion, and the reinvestigation of its host specificity. The genome consists of a linear, double-stranded 148,612-bp DNA sequence containing 248 potential open reading frames and 11 putative tRNA genes. Orthologs were found for 130 of the predicted proteins. Most of the virion proteins showed significant sequence similarities to proteins of myoviruses rv5 and PVP-SE1, indicating that phi92 is a new member of the novel genus of rv5-like phages. Reinvestigation of phi92 host specificity showed that the host range is not limited to polysialic acid-encapsulated Escherichia coli but includes most laboratory strains of Escherichia coli and many Salmonella strains. Structure analysis of the phi92 virion demonstrated the presence of four different types of tail fibers and/or tailspikes, which enable the phage to use attachment sites on encapsulated and nonencapsulated bacteria. With this report, we provide the first detailed description of a multivalent, multispecies phage armed with a host cell adsorption apparatus resembling a nanosized Swiss army knife. The genome, structure, and, in particular, the organization of the baseplate of phi92 demonstrate how a bacteriophage can evolve into a multi-pathogen-killing agent.

Original languageEnglish (US)
Pages (from-to)10384-10398
Number of pages15
JournalJournal of Virology
Volume86
Issue number19
DOIs
StatePublished - Oct 2012
Externally publishedYes

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Host Specificity
host range
bacteriophages
Bacteriophages
Adsorption
adsorption
genomics
virion
Virion
Genome
host specificity
Escherichia coli
genome
Cryoelectron Microscopy
Proteins
knives
proteins
acids
Transfer RNA
Salmonella

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Schwarzer, D., Buettner, F. F. R., Browning, C., Nazarov, S., Rabsch, W., Bethe, A., ... Gerardy-Schahn, R. (2012). A multivalent adsorption apparatus explains the broad host range of phage phi92: A comprehensive genomic and structural analysis. Journal of Virology, 86(19), 10384-10398. https://doi.org/10.1128/JVI.00801-12

A multivalent adsorption apparatus explains the broad host range of phage phi92 : A comprehensive genomic and structural analysis. / Schwarzer, David; Buettner, Falk F R; Browning, Christopher; Nazarov, Sergey; Rabsch, Wolfgang; Bethe, Andrea; Oberbeck, Astrid; Bowman, Valorie D.; Stummeyer, Katharina; Mühlenhoff, Martina; Leiman, Petr; Gerardy-Schahn, Rita.

In: Journal of Virology, Vol. 86, No. 19, 10.2012, p. 10384-10398.

Research output: Contribution to journalArticle

Schwarzer, D, Buettner, FFR, Browning, C, Nazarov, S, Rabsch, W, Bethe, A, Oberbeck, A, Bowman, VD, Stummeyer, K, Mühlenhoff, M, Leiman, P & Gerardy-Schahn, R 2012, 'A multivalent adsorption apparatus explains the broad host range of phage phi92: A comprehensive genomic and structural analysis', Journal of Virology, vol. 86, no. 19, pp. 10384-10398. https://doi.org/10.1128/JVI.00801-12
Schwarzer, David ; Buettner, Falk F R ; Browning, Christopher ; Nazarov, Sergey ; Rabsch, Wolfgang ; Bethe, Andrea ; Oberbeck, Astrid ; Bowman, Valorie D. ; Stummeyer, Katharina ; Mühlenhoff, Martina ; Leiman, Petr ; Gerardy-Schahn, Rita. / A multivalent adsorption apparatus explains the broad host range of phage phi92 : A comprehensive genomic and structural analysis. In: Journal of Virology. 2012 ; Vol. 86, No. 19. pp. 10384-10398.
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