A murine vascular endothelial growth factor antibody inhibits in vivo growth of human Caki-I renal adenocarcinoma

F. Dagnaes-Hansen, L. M. Rasmussen, R. Tilton, L. Denner, A. Flyvbjerg

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

A number of studies have provided indirect evidence that angiogenesis is involved in tumour growth and metastasis formation of renal cell carcinoma (RCC). Nude mice bearing xenografts of human Caki-I RCC were treated i.p. for 3 weeks with a murine monoclonal antibody against vascular endothelial growth factor, VEGF (VEGF-ab). Tumour growth and mRNA expression of human and murine VEGF and murine VE-Cadherin in the tumours were measured. After 3 weeks of therapy, the tumour volume in the control nude mice was 548 ± 98 mm3 compared to the tumours in the nude mice treated with VEGF-ab (122 ± 24 mm3, p < 0.01). Treatment with VEGF-ab significantly reduced mRNA expression of murine VEGF-120 and murine VE-Cadherin (p < 0.05 and p < 0.01, respectively). The mRNA expression of human VEGF (hVEGF165, hVEGF189) and murine VEGF (mVEGF164) was unchanged due to antibody treatment. The mean percentage of apoptotic cells in tumours harvested from antibody-treated animals was significantly lower than in tumours from the control-treated animals (p < 0.02). These findings demonstrate for the first time that VEGF-ab significantly inhibit the growth of Caki-I RCC in vivo.

Original languageEnglish (US)
Pages (from-to)1625-1630
Number of pages6
JournalAnticancer Research
Volume23
Issue number2 B
StatePublished - Mar 1 2003

Keywords

  • Experimental kidney neoplasms
  • mRNA
  • Renal cell carcinoma
  • Vascular endothelial growth factor
  • VE-Cadherin
  • VEGF

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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