Abstract
In this improved method of embedding tissue culture specimens, n-butylglycidyl ether is added to fixed, dehydrated cells within the plastic culture flasks. The n-butylglycidyl ether dissolves the flask, allowing a sheet of cells and a microscopic remnant of the plastic culture flask to separate from the culture dish and float freely on the surface of the ether. The layer of cells can then be folded or centrifuged; and upon examination the flask remnant can be located microscopically to determine orientation. This new method provides excellent morphology, greatly increased sample size, and marked reduction in processing time.
Original language | English (US) |
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Pages (from-to) | 223-226 |
Number of pages | 4 |
Journal | Journal of Tissue Culture Methods |
Volume | 10 |
Issue number | 4 |
DOIs | |
State | Published - Dec 1986 |
Externally published | Yes |
Keywords
- floating sheet
- n-butylglycidyl ether
- tissue culture
- transmission EM
ASJC Scopus subject areas
- Anatomy
- Pathology and Forensic Medicine