A new method of processing cultured cells for transmission electron microscopy: The floating sheet

James R. Arnold, Paul J. Boor

Research output: Contribution to journalArticle

Abstract

In this improved method of embedding tissue culture specimens, n-butylglycidyl ether is added to fixed, dehydrated cells within the plastic culture flasks. The n-butylglycidyl ether dissolves the flask, allowing a sheet of cells and a microscopic remnant of the plastic culture flask to separate from the culture dish and float freely on the surface of the ether. The layer of cells can then be folded or centrifuged; and upon examination the flask remnant can be located microscopically to determine orientation. This new method provides excellent morphology, greatly increased sample size, and marked reduction in processing time.

Original languageEnglish (US)
Pages (from-to)223-226
Number of pages4
JournalJournal of Tissue Culture Methods
Volume10
Issue number4
DOIs
StatePublished - Dec 1986

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Keywords

  • floating sheet
  • n-butylglycidyl ether
  • tissue culture
  • transmission EM

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Anatomy

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