A newly identified pattern of K-ras mutations at codons 12 and 13 is associated with long-term survival in colorectal cancer

Anthony J. Senagore, Jennifer Thebo Biener

    Research output: Contribution to journalArticle

    15 Scopus citations

    Abstract

    Background. Although K-ras mutations reportedly occur in 40% to 60% of all colorectal carcinomas, the relationship between specific mutations and clinical outcome is unclear. The purpose of this study was to assess the frequency and types of K-ras mutations in 89 colorectal cancer patients, comparing groups with short-term (less than 5 years) and long-term (more than 10 years) survival. Methods. The group was divided into four cohorts by survival and modified Dukes classification (Dukes B2 and C2). DNA was extracted from formalin-fixed paraffin-embedded archival material. Mutational status was analyzed using a modification of allele-specific-polymerase chain reaction. Results. Mutations in codon 12 were found in 11.2% of tumors, and 83% of tumors had mutations in codon 13. Gly > Asp accounted for 85.2% of the mutations. Tumors with mutations in both codon 12 and codon 13 occurred significantly more frequently in the long-term (21.3%) versus the short-term (2.4%) survival group. Gly > Asp mutations in either codon were related to long-term survival, and 80% of long-term survivors with mutations in both codons had Gly > Asp mutations in both. Conclusions. Simultaneous mutation in codons 12 and 13 of the K-ras gene appears to be a positive prognostic indicator in colorectal cancer.

    Original languageEnglish (US)
    Pages (from-to)765-770
    Number of pages6
    JournalSurgery
    Volume122
    Issue number4
    DOIs
    StatePublished - Oct 1997

    ASJC Scopus subject areas

    • Surgery

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