A newly isolated reovirus has the simplest genomic and structural organization of any reovirus

Albert J. Auguste, Jason T. Kaelber, Eric B. Fokam, Hilda Guzman, Christine V F Carrington, Jesse H. Erasmus, Basile Kamgang, Vsevolod Popov, Joanita Jakana, Xiangan Liu, Thomas Wood, Steven Widen, Nikos Vasilakis, Robert B. Tesh, Wah Chiu, Scott Weaver

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A total of 2,691 mosquitoes representing 17 species was collected from eight locations in southwest Cameroon and screened for pathogenic viruses. Ten isolates of a novel reovirus (genus Dinovernavirus) were detected by culturing mosquito pools on Aedes albopictus (C6/36) cell cultures. A virus that caused overt cytopathic effects was isolated, but it did not infect vertebrate cells or produce detectable disease in infant mice after intracerebral inoculation. The virus, tentatively designated Fako virus (FAKV), represents the first 9-segment, double-stranded RNA (dsRNA) virus to be isolated in nature. FAKV appears to have a broad mosquito host range, and its detection in male specimens suggests mosquito-to-mosquito transmission in nature. The structure of the T=1 FAKV virion, determined to subnanometer resolution by cryoelectron microscopy (cryo-EM), showed only four proteins per icosahedral asymmetric unit: a dimer of the major capsid protein, one turret protein, and one clamp protein. While all other turreted reoviruses of known structures have at least two copies of the clamp protein per asymmetric unit, FAKV's clamp protein bound at only one conformer of the major capsid protein. The FAKV capsid architecture and genome organization represent the most simplified reovirus described to date, and phylogenetic analysis suggests that it arose from a more complex ancestor by serial loss-of-function events.

Original languageEnglish (US)
Pages (from-to)676-687
Number of pages12
JournalJournal of Virology
Volume89
Issue number1
DOIs
StatePublished - 2015

Fingerprint

Reoviridae
Culicidae
Viruses
genomics
viruses
Capsid Proteins
Proteins
coat proteins
proteins
dsRNA viruses
Cryoelectron Microscopy
Cameroon
cytopathogenicity
capsid
Double-Stranded RNA
Aedes albopictus
Aedes
Capsid
Host Specificity
RNA Viruses

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Auguste, A. J., Kaelber, J. T., Fokam, E. B., Guzman, H., Carrington, C. V. F., Erasmus, J. H., ... Weaver, S. (2015). A newly isolated reovirus has the simplest genomic and structural organization of any reovirus. Journal of Virology, 89(1), 676-687. https://doi.org/10.1128/JVI.02264-14

A newly isolated reovirus has the simplest genomic and structural organization of any reovirus. / Auguste, Albert J.; Kaelber, Jason T.; Fokam, Eric B.; Guzman, Hilda; Carrington, Christine V F; Erasmus, Jesse H.; Kamgang, Basile; Popov, Vsevolod; Jakana, Joanita; Liu, Xiangan; Wood, Thomas; Widen, Steven; Vasilakis, Nikos; Tesh, Robert B.; Chiu, Wah; Weaver, Scott.

In: Journal of Virology, Vol. 89, No. 1, 2015, p. 676-687.

Research output: Contribution to journalArticle

Auguste, AJ, Kaelber, JT, Fokam, EB, Guzman, H, Carrington, CVF, Erasmus, JH, Kamgang, B, Popov, V, Jakana, J, Liu, X, Wood, T, Widen, S, Vasilakis, N, Tesh, RB, Chiu, W & Weaver, S 2015, 'A newly isolated reovirus has the simplest genomic and structural organization of any reovirus', Journal of Virology, vol. 89, no. 1, pp. 676-687. https://doi.org/10.1128/JVI.02264-14
Auguste AJ, Kaelber JT, Fokam EB, Guzman H, Carrington CVF, Erasmus JH et al. A newly isolated reovirus has the simplest genomic and structural organization of any reovirus. Journal of Virology. 2015;89(1):676-687. https://doi.org/10.1128/JVI.02264-14
Auguste, Albert J. ; Kaelber, Jason T. ; Fokam, Eric B. ; Guzman, Hilda ; Carrington, Christine V F ; Erasmus, Jesse H. ; Kamgang, Basile ; Popov, Vsevolod ; Jakana, Joanita ; Liu, Xiangan ; Wood, Thomas ; Widen, Steven ; Vasilakis, Nikos ; Tesh, Robert B. ; Chiu, Wah ; Weaver, Scott. / A newly isolated reovirus has the simplest genomic and structural organization of any reovirus. In: Journal of Virology. 2015 ; Vol. 89, No. 1. pp. 676-687.
@article{11a2493f8d584239b95f876a1bc2efb6,
title = "A newly isolated reovirus has the simplest genomic and structural organization of any reovirus",
abstract = "A total of 2,691 mosquitoes representing 17 species was collected from eight locations in southwest Cameroon and screened for pathogenic viruses. Ten isolates of a novel reovirus (genus Dinovernavirus) were detected by culturing mosquito pools on Aedes albopictus (C6/36) cell cultures. A virus that caused overt cytopathic effects was isolated, but it did not infect vertebrate cells or produce detectable disease in infant mice after intracerebral inoculation. The virus, tentatively designated Fako virus (FAKV), represents the first 9-segment, double-stranded RNA (dsRNA) virus to be isolated in nature. FAKV appears to have a broad mosquito host range, and its detection in male specimens suggests mosquito-to-mosquito transmission in nature. The structure of the T=1 FAKV virion, determined to subnanometer resolution by cryoelectron microscopy (cryo-EM), showed only four proteins per icosahedral asymmetric unit: a dimer of the major capsid protein, one turret protein, and one clamp protein. While all other turreted reoviruses of known structures have at least two copies of the clamp protein per asymmetric unit, FAKV's clamp protein bound at only one conformer of the major capsid protein. The FAKV capsid architecture and genome organization represent the most simplified reovirus described to date, and phylogenetic analysis suggests that it arose from a more complex ancestor by serial loss-of-function events.",
author = "Auguste, {Albert J.} and Kaelber, {Jason T.} and Fokam, {Eric B.} and Hilda Guzman and Carrington, {Christine V F} and Erasmus, {Jesse H.} and Basile Kamgang and Vsevolod Popov and Joanita Jakana and Xiangan Liu and Thomas Wood and Steven Widen and Nikos Vasilakis and Tesh, {Robert B.} and Wah Chiu and Scott Weaver",
year = "2015",
doi = "10.1128/JVI.02264-14",
language = "English (US)",
volume = "89",
pages = "676--687",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - A newly isolated reovirus has the simplest genomic and structural organization of any reovirus

AU - Auguste, Albert J.

AU - Kaelber, Jason T.

AU - Fokam, Eric B.

AU - Guzman, Hilda

AU - Carrington, Christine V F

AU - Erasmus, Jesse H.

AU - Kamgang, Basile

AU - Popov, Vsevolod

AU - Jakana, Joanita

AU - Liu, Xiangan

AU - Wood, Thomas

AU - Widen, Steven

AU - Vasilakis, Nikos

AU - Tesh, Robert B.

AU - Chiu, Wah

AU - Weaver, Scott

PY - 2015

Y1 - 2015

N2 - A total of 2,691 mosquitoes representing 17 species was collected from eight locations in southwest Cameroon and screened for pathogenic viruses. Ten isolates of a novel reovirus (genus Dinovernavirus) were detected by culturing mosquito pools on Aedes albopictus (C6/36) cell cultures. A virus that caused overt cytopathic effects was isolated, but it did not infect vertebrate cells or produce detectable disease in infant mice after intracerebral inoculation. The virus, tentatively designated Fako virus (FAKV), represents the first 9-segment, double-stranded RNA (dsRNA) virus to be isolated in nature. FAKV appears to have a broad mosquito host range, and its detection in male specimens suggests mosquito-to-mosquito transmission in nature. The structure of the T=1 FAKV virion, determined to subnanometer resolution by cryoelectron microscopy (cryo-EM), showed only four proteins per icosahedral asymmetric unit: a dimer of the major capsid protein, one turret protein, and one clamp protein. While all other turreted reoviruses of known structures have at least two copies of the clamp protein per asymmetric unit, FAKV's clamp protein bound at only one conformer of the major capsid protein. The FAKV capsid architecture and genome organization represent the most simplified reovirus described to date, and phylogenetic analysis suggests that it arose from a more complex ancestor by serial loss-of-function events.

AB - A total of 2,691 mosquitoes representing 17 species was collected from eight locations in southwest Cameroon and screened for pathogenic viruses. Ten isolates of a novel reovirus (genus Dinovernavirus) were detected by culturing mosquito pools on Aedes albopictus (C6/36) cell cultures. A virus that caused overt cytopathic effects was isolated, but it did not infect vertebrate cells or produce detectable disease in infant mice after intracerebral inoculation. The virus, tentatively designated Fako virus (FAKV), represents the first 9-segment, double-stranded RNA (dsRNA) virus to be isolated in nature. FAKV appears to have a broad mosquito host range, and its detection in male specimens suggests mosquito-to-mosquito transmission in nature. The structure of the T=1 FAKV virion, determined to subnanometer resolution by cryoelectron microscopy (cryo-EM), showed only four proteins per icosahedral asymmetric unit: a dimer of the major capsid protein, one turret protein, and one clamp protein. While all other turreted reoviruses of known structures have at least two copies of the clamp protein per asymmetric unit, FAKV's clamp protein bound at only one conformer of the major capsid protein. The FAKV capsid architecture and genome organization represent the most simplified reovirus described to date, and phylogenetic analysis suggests that it arose from a more complex ancestor by serial loss-of-function events.

UR - http://www.scopus.com/inward/record.url?scp=84919458611&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84919458611&partnerID=8YFLogxK

U2 - 10.1128/JVI.02264-14

DO - 10.1128/JVI.02264-14

M3 - Article

VL - 89

SP - 676

EP - 687

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 1

ER -