The gene encoding a nontoxic analog, CT-2* of cholera enterotoxin (CT) with attenuating codon substitutions in the A subunit was introduced into the attenuated Vibrio cholerae classical biotype mutant candidate vaccine strain CVD103, which produces the B subunit (but nut the A subunit) of CT-1. The recombinant strain produces a chimeric nontoxic analog holotoxin protein containing both CT-B-1 and CT-B-2 subunits. This offers potential advantages over CVD103 in the induction of immunity against El Tor biotype and V. cholerae O139 strains which produce CT-B-2. The recombinant protein may also be useful in polysaccharide-protein conjugate vaccines against both O1 and O139 serovars of V. cholerae.
|Original language||English (US)|
|Number of pages||3|
|Journal||Infection and Immunity|
|State||Published - 1996|
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