A novel acid-sensitive K+ channel in rat dorsal root ganglia neurons

Jun-Ho La, Dawon Kang, Jae Yong Park, Seong Geun Hong, Jaehee Han

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Recent studies have suggested that acid-sensitive background K+ channels such as TASK-1 and TASK-3, members of two-pore domain K+ (K2P) channel family, express and contribute to extracellular acidification-induced responses in dorsal root ganglia (DRG) neurons. However, it has remained to address whether other acid-sensitive background K+ channels are functionally expressed in DRG neurons. Here we characterized biophysical and pharmacological properties of a novel acid-sensitive background K+ channel in DRG neurons isolated from neonatal rats. We recorded an 80-pS K+ channel with a weak inward rectification current-voltage relationship in cell-attached patches in 150 mM KCl bath solution. The 80-pS K+ channel was inhibited by extracellular low pH (pHo 6.3). Interestingly, the channel was similar to TASK-2 cloned from mouse and rat in biophysical and pharmacological properties. However, extracellular alkaline condition which activates TASK-2 channel, failed to activate the 80-pS K+ channel. Lidocaine and quinine more inhibited the channel activity of 80-pS K+ channel than that of TASK-2 channel. Our results suggest that the acid-sensitive 80-pS K+ channels may regulate resting membrane potential and may play a critical role in various processes such as cell metabolism, pH, and pain sensation in DRG neurons.

Original languageEnglish (US)
Pages (from-to)244-249
Number of pages6
JournalNeuroscience Letters
Volume406
Issue number3
DOIs
StatePublished - Oct 9 2006
Externally publishedYes

Fingerprint

Spinal Ganglia
Neurons
Acids
Pharmacology
Quinine
Lidocaine
Baths
Membrane Potentials
Pain

Keywords

  • Acid-sensitive channel
  • Background K channels
  • Dorsal root ganglion neurons
  • TASK-2

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

A novel acid-sensitive K+ channel in rat dorsal root ganglia neurons. / La, Jun-Ho; Kang, Dawon; Park, Jae Yong; Hong, Seong Geun; Han, Jaehee.

In: Neuroscience Letters, Vol. 406, No. 3, 09.10.2006, p. 244-249.

Research output: Contribution to journalArticle

La, Jun-Ho ; Kang, Dawon ; Park, Jae Yong ; Hong, Seong Geun ; Han, Jaehee. / A novel acid-sensitive K+ channel in rat dorsal root ganglia neurons. In: Neuroscience Letters. 2006 ; Vol. 406, No. 3. pp. 244-249.
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