A novel animal model of sepsis after acute lung injury in sheep

Kazunori Murakami, Lars J. Bjertnaes, Frank C. Schmalstieg, Roy McGuire, Robert A. Cox, Hal K. Hawkins, David Herndon, Lillian D. Traber, Daniel L. Traber

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Objective: Patients with acute lung injury after smoke inhalation often develop pneumonia subsequently complicated by sepsis. This often is a fatal complication. The aim of this study was to develop a standardized and reproducible model of hyperdynamic sepsis after smoke inhalation in sheep. Design: Prospective, experimental study in sheep. Settings: Experimental laboratory in a university hospital. Subjects: Twenty-one female Merino ewes. Intervention: Animals were anesthetized and surgically prepared for this chronic study. After a week of recovery, baseline data were collected. After tracheostomy was performed, sheep were connected to a volume-controlled ventilator. Acute lung injury was produced by insufflating the lungs with 48 breaths of cotton smoke. During halothane anesthesia, live Pseudomonas aeruginosa bacteria suspended in a 30-mL saline solution containing 2-5 × 1011 colony-forming units were instilled through a bronchoscope into the right lower and middle lung lobes (10 mL each) and left lower lung lobe (10 mL; n = 10). Eleven sheep were given smoke but not bacteria. After injury and the bacterial challenge, the animals were ventilated mechanically with 100% oxygen. The animals were monitored for 48 hrs. P. aeruginosa was detected in blood cultures after 14-48 hrs. Measurements and Main Results: The sheep developed a hyperkinetic cardiovascular response concomitant with a decrease in Pao2 similar to severe sepsis in human patients who meet the criteria for acute respiratory distress syndrome (Pao2/Flo2 <200). These changes were more severe than in animals exposed to smoke inhalation alone. Mean arterial pressures at 48 hrs in the smoke-alone and the smoke + sepsis group were 85.5 ± 5.2 and 68.1 ± 7.6 mm Hg, respectively (mean ± SE, p < .05). Conclusion: This animal model closely resembles hyperdynamic sepsis in humans and may be of great value for studies of sepsis with smoke inhalation.

Original languageEnglish (US)
Pages (from-to)2083-2090
Number of pages8
JournalCritical Care Medicine
Volume30
Issue number9
StatePublished - Sep 1 2002

Fingerprint

Acute Lung Injury
Smoke
Sheep
Sepsis
Animal Models
Inhalation
Lung
Pseudomonas aeruginosa
Bacteria
Bronchoscopes
Tracheostomy
Adult Respiratory Distress Syndrome
Halothane
Mechanical Ventilators
Sodium Chloride
Pneumonia
Arterial Pressure
Research Design
Stem Cells
Anesthesia

Keywords

  • Acute lung injury
  • Acute respiratory distress syndrome
  • Airway cast
  • Histology
  • Hyperdynamic
  • Nitric oxide
  • Pneumonia
  • Pseudomonas aeruginosa
  • Sheep
  • Shock
  • Smoke inhalation

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Murakami, K., Bjertnaes, L. J., Schmalstieg, F. C., McGuire, R., Cox, R. A., Hawkins, H. K., ... Traber, D. L. (2002). A novel animal model of sepsis after acute lung injury in sheep. Critical Care Medicine, 30(9), 2083-2090.

A novel animal model of sepsis after acute lung injury in sheep. / Murakami, Kazunori; Bjertnaes, Lars J.; Schmalstieg, Frank C.; McGuire, Roy; Cox, Robert A.; Hawkins, Hal K.; Herndon, David; Traber, Lillian D.; Traber, Daniel L.

In: Critical Care Medicine, Vol. 30, No. 9, 01.09.2002, p. 2083-2090.

Research output: Contribution to journalArticle

Murakami, K, Bjertnaes, LJ, Schmalstieg, FC, McGuire, R, Cox, RA, Hawkins, HK, Herndon, D, Traber, LD & Traber, DL 2002, 'A novel animal model of sepsis after acute lung injury in sheep', Critical Care Medicine, vol. 30, no. 9, pp. 2083-2090.
Murakami K, Bjertnaes LJ, Schmalstieg FC, McGuire R, Cox RA, Hawkins HK et al. A novel animal model of sepsis after acute lung injury in sheep. Critical Care Medicine. 2002 Sep 1;30(9):2083-2090.
Murakami, Kazunori ; Bjertnaes, Lars J. ; Schmalstieg, Frank C. ; McGuire, Roy ; Cox, Robert A. ; Hawkins, Hal K. ; Herndon, David ; Traber, Lillian D. ; Traber, Daniel L. / A novel animal model of sepsis after acute lung injury in sheep. In: Critical Care Medicine. 2002 ; Vol. 30, No. 9. pp. 2083-2090.
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abstract = "Objective: Patients with acute lung injury after smoke inhalation often develop pneumonia subsequently complicated by sepsis. This often is a fatal complication. The aim of this study was to develop a standardized and reproducible model of hyperdynamic sepsis after smoke inhalation in sheep. Design: Prospective, experimental study in sheep. Settings: Experimental laboratory in a university hospital. Subjects: Twenty-one female Merino ewes. Intervention: Animals were anesthetized and surgically prepared for this chronic study. After a week of recovery, baseline data were collected. After tracheostomy was performed, sheep were connected to a volume-controlled ventilator. Acute lung injury was produced by insufflating the lungs with 48 breaths of cotton smoke. During halothane anesthesia, live Pseudomonas aeruginosa bacteria suspended in a 30-mL saline solution containing 2-5 × 1011 colony-forming units were instilled through a bronchoscope into the right lower and middle lung lobes (10 mL each) and left lower lung lobe (10 mL; n = 10). Eleven sheep were given smoke but not bacteria. After injury and the bacterial challenge, the animals were ventilated mechanically with 100{\%} oxygen. The animals were monitored for 48 hrs. P. aeruginosa was detected in blood cultures after 14-48 hrs. Measurements and Main Results: The sheep developed a hyperkinetic cardiovascular response concomitant with a decrease in Pao2 similar to severe sepsis in human patients who meet the criteria for acute respiratory distress syndrome (Pao2/Flo2 <200). These changes were more severe than in animals exposed to smoke inhalation alone. Mean arterial pressures at 48 hrs in the smoke-alone and the smoke + sepsis group were 85.5 ± 5.2 and 68.1 ± 7.6 mm Hg, respectively (mean ± SE, p < .05). Conclusion: This animal model closely resembles hyperdynamic sepsis in humans and may be of great value for studies of sepsis with smoke inhalation.",
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AU - Bjertnaes, Lars J.

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AU - Cox, Robert A.

AU - Hawkins, Hal K.

AU - Herndon, David

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N2 - Objective: Patients with acute lung injury after smoke inhalation often develop pneumonia subsequently complicated by sepsis. This often is a fatal complication. The aim of this study was to develop a standardized and reproducible model of hyperdynamic sepsis after smoke inhalation in sheep. Design: Prospective, experimental study in sheep. Settings: Experimental laboratory in a university hospital. Subjects: Twenty-one female Merino ewes. Intervention: Animals were anesthetized and surgically prepared for this chronic study. After a week of recovery, baseline data were collected. After tracheostomy was performed, sheep were connected to a volume-controlled ventilator. Acute lung injury was produced by insufflating the lungs with 48 breaths of cotton smoke. During halothane anesthesia, live Pseudomonas aeruginosa bacteria suspended in a 30-mL saline solution containing 2-5 × 1011 colony-forming units were instilled through a bronchoscope into the right lower and middle lung lobes (10 mL each) and left lower lung lobe (10 mL; n = 10). Eleven sheep were given smoke but not bacteria. After injury and the bacterial challenge, the animals were ventilated mechanically with 100% oxygen. The animals were monitored for 48 hrs. P. aeruginosa was detected in blood cultures after 14-48 hrs. Measurements and Main Results: The sheep developed a hyperkinetic cardiovascular response concomitant with a decrease in Pao2 similar to severe sepsis in human patients who meet the criteria for acute respiratory distress syndrome (Pao2/Flo2 <200). These changes were more severe than in animals exposed to smoke inhalation alone. Mean arterial pressures at 48 hrs in the smoke-alone and the smoke + sepsis group were 85.5 ± 5.2 and 68.1 ± 7.6 mm Hg, respectively (mean ± SE, p < .05). Conclusion: This animal model closely resembles hyperdynamic sepsis in humans and may be of great value for studies of sepsis with smoke inhalation.

AB - Objective: Patients with acute lung injury after smoke inhalation often develop pneumonia subsequently complicated by sepsis. This often is a fatal complication. The aim of this study was to develop a standardized and reproducible model of hyperdynamic sepsis after smoke inhalation in sheep. Design: Prospective, experimental study in sheep. Settings: Experimental laboratory in a university hospital. Subjects: Twenty-one female Merino ewes. Intervention: Animals were anesthetized and surgically prepared for this chronic study. After a week of recovery, baseline data were collected. After tracheostomy was performed, sheep were connected to a volume-controlled ventilator. Acute lung injury was produced by insufflating the lungs with 48 breaths of cotton smoke. During halothane anesthesia, live Pseudomonas aeruginosa bacteria suspended in a 30-mL saline solution containing 2-5 × 1011 colony-forming units were instilled through a bronchoscope into the right lower and middle lung lobes (10 mL each) and left lower lung lobe (10 mL; n = 10). Eleven sheep were given smoke but not bacteria. After injury and the bacterial challenge, the animals were ventilated mechanically with 100% oxygen. The animals were monitored for 48 hrs. P. aeruginosa was detected in blood cultures after 14-48 hrs. Measurements and Main Results: The sheep developed a hyperkinetic cardiovascular response concomitant with a decrease in Pao2 similar to severe sepsis in human patients who meet the criteria for acute respiratory distress syndrome (Pao2/Flo2 <200). These changes were more severe than in animals exposed to smoke inhalation alone. Mean arterial pressures at 48 hrs in the smoke-alone and the smoke + sepsis group were 85.5 ± 5.2 and 68.1 ± 7.6 mm Hg, respectively (mean ± SE, p < .05). Conclusion: This animal model closely resembles hyperdynamic sepsis in humans and may be of great value for studies of sepsis with smoke inhalation.

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KW - Airway cast

KW - Histology

KW - Hyperdynamic

KW - Nitric oxide

KW - Pneumonia

KW - Pseudomonas aeruginosa

KW - Sheep

KW - Shock

KW - Smoke inhalation

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