A Novel Approach for Intestinal Elongation Using Acellular Dermal Matrix

An Experimental Study in Rats

M. P. Pahari, A. Raman, A. Bloomenthal, M. A. Costa, S. P. Bradley, B. Banner, Cristiana Rastellini, Luca Cicalese

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We tested the hypothesis that an anatomic scaffold placed in continuity with viable bowel might allow intestinal growth. Male ACI rats were used for the study. Acellular human dermis in the form of tubular scaffolds with an intraluminal diameter of approximately 0.3 cm was oriented with the luminal basement membrane and serosal dermal surface. The small bowel was transected approximately 2 cm distal to the ligament of Treitz. The graft was then anastomosed in continuity in group A (n = 5) or as a blind-ended pouch to a defunctionalized jejunal limb in group B (n = 8). The animals were sacrificed at various time points. Histology and immunohistochemistry were used to evaluate structural changes. Animals in group A developed peritonitis and were all sacrificed within the first week postoperatively. However, all animals in group B survived, increasing their body weight similarly to age-matched rats. Tissue samples obtained at sacrifice showed a progressively increasing amount of cellular infiltrate over time in the matrix. Epithelial regeneration, angioneogenesis, and myofibroblast infiltrate were seen at 2 weeks, while well-formed branching crypts were seen at 4 weeks. Intact mucosa extended across the anastomosis to the grafts at 6 months. This study demonstrated an anatomic scaffold of acellular matrix allowed mucosal regeneration from viable bowel placed in continuity. These findings set the basis for new intestinal elongation techniques.

Original languageEnglish (US)
Pages (from-to)1849-1850
Number of pages2
JournalTransplantation Proceedings
Volume38
Issue number6
DOIs
StatePublished - Jul 2006
Externally publishedYes

Fingerprint

Acellular Dermis
Regeneration
Inbred ACI Rats
Transplants
Myofibroblasts
Peritonitis
Ligaments
Basement Membrane
Histology
Mucous Membrane
Extremities
Immunohistochemistry
Body Weight
Skin
Growth

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Cite this

A Novel Approach for Intestinal Elongation Using Acellular Dermal Matrix : An Experimental Study in Rats. / Pahari, M. P.; Raman, A.; Bloomenthal, A.; Costa, M. A.; Bradley, S. P.; Banner, B.; Rastellini, Cristiana; Cicalese, Luca.

In: Transplantation Proceedings, Vol. 38, No. 6, 07.2006, p. 1849-1850.

Research output: Contribution to journalArticle

Pahari, MP, Raman, A, Bloomenthal, A, Costa, MA, Bradley, SP, Banner, B, Rastellini, C & Cicalese, L 2006, 'A Novel Approach for Intestinal Elongation Using Acellular Dermal Matrix: An Experimental Study in Rats', Transplantation Proceedings, vol. 38, no. 6, pp. 1849-1850. https://doi.org/10.1016/j.transproceed.2006.05.052
Pahari, M. P. ; Raman, A. ; Bloomenthal, A. ; Costa, M. A. ; Bradley, S. P. ; Banner, B. ; Rastellini, Cristiana ; Cicalese, Luca. / A Novel Approach for Intestinal Elongation Using Acellular Dermal Matrix : An Experimental Study in Rats. In: Transplantation Proceedings. 2006 ; Vol. 38, No. 6. pp. 1849-1850.
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