A novel approach for screening the proteome for changes in protein conformation

Anson Pierce, Eric DeWaal, Holly VanRemmen, Arlan Richardson, Asish Chaudhuri

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Changes in surface hydrophobicity are generally considered as a sensitive indicator for monitoring the structural alterations of proteins that are often associated with changes in function. Currently, no technique has been developed to screen a complex mixture of proteins for changes in the conformation of specific proteins. In this study, we adapted a UV photolabeling approach, using an apolar fluorescent probe, 4,4′-dianilino-1,1′-binaphthyl-5, 5′-disulfonic acid (BisANS), to monitor changes in surface hydrophobic domains in either purified rhodanese or skeletal muscle cytosolic proteins by urea-induced unfolding or in response to in vitro metal-catalyzed oxidation. Using two-dimensional polyacrylamide gel electrophoresis (2D PAGE), we identified two specific proteins in skeletal muscle cytosol that exhibited a marked loss of incorporation of BisANS after exposure to in vitro oxidative stress: creatine kinase (CK) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). We found that the activities of both enzymes were also reduced significantly in response to oxidative stress. We then determined if this method could detect changes in surface hydrophobicity in specific proteins arising from oxidative stress generated in vivo by muscle denervation. A loss in surface hydrophobic domains in CK and GAPDH was again observed as measured by the BisANS photoincorporation approach. In addition, the CK and GAPDH activity in denervated muscle was markedly reduced. These data demonstrate for the first time that this assay can screen a complex mixture of proteins for alterations in surface hydrophobic domains of individual proteins.

Original languageEnglish (US)
Pages (from-to)3077-3085
Number of pages9
JournalBiochemistry
Volume45
Issue number9
DOIs
StatePublished - Mar 7 2006
Externally publishedYes

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Protein Conformation
Proteome
Conformations
Screening
Glyceraldehyde-3-Phosphate Dehydrogenases
Creatine Kinase
Oxidative Stress
Oxidative stress
Electrophoresis, Gel, Two-Dimensional
Muscle
Proteins
Complex Mixtures
Hydrophobic and Hydrophilic Interactions
Skeletal Muscle
Thiosulfate Sulfurtransferase
Muscle Denervation
Hydrophobicity
Muscle Proteins
Fluorescent Dyes
Cytosol

ASJC Scopus subject areas

  • Biochemistry

Cite this

Pierce, A., DeWaal, E., VanRemmen, H., Richardson, A., & Chaudhuri, A. (2006). A novel approach for screening the proteome for changes in protein conformation. Biochemistry, 45(9), 3077-3085. https://doi.org/10.1021/bi052031i

A novel approach for screening the proteome for changes in protein conformation. / Pierce, Anson; DeWaal, Eric; VanRemmen, Holly; Richardson, Arlan; Chaudhuri, Asish.

In: Biochemistry, Vol. 45, No. 9, 07.03.2006, p. 3077-3085.

Research output: Contribution to journalArticle

Pierce, A, DeWaal, E, VanRemmen, H, Richardson, A & Chaudhuri, A 2006, 'A novel approach for screening the proteome for changes in protein conformation', Biochemistry, vol. 45, no. 9, pp. 3077-3085. https://doi.org/10.1021/bi052031i
Pierce A, DeWaal E, VanRemmen H, Richardson A, Chaudhuri A. A novel approach for screening the proteome for changes in protein conformation. Biochemistry. 2006 Mar 7;45(9):3077-3085. https://doi.org/10.1021/bi052031i
Pierce, Anson ; DeWaal, Eric ; VanRemmen, Holly ; Richardson, Arlan ; Chaudhuri, Asish. / A novel approach for screening the proteome for changes in protein conformation. In: Biochemistry. 2006 ; Vol. 45, No. 9. pp. 3077-3085.
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