A Novel Approach to Collision-Induced Dissociation (CID) for Ion Mobility-Mass Spectrometry Experiments

Christopher Becker, Francisco A. Fernandez-Lima, Kent J. Gillig, William Russell, Stephanie M. Cologna, David H. Russell

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Collision induced dissociation (CID) combined with matrix assisted laser desorption ionization-ion mobility-mass spectrometry (MALDI-IM-MS) is described. In this approach, peptide ions are separated on the basis of mobility in a 15 cm drift cell. Following mobility separation, the ions exit the drift cell and enter a 5 cm vacuum interface with a high field region (up to 1000 V/cm) to undergo collisional activation. Ion transmission and ion kinetic energies in the interface are theoretically evaluated accounting for the pressure gradient, interface dimensions, and electric fields. Using this CID technique, we have successfully fragmented and sequenced a number of model peptide ions as well as peptide ions obtained by a tryptic digest. This instrument configuration allows for the simultaneous determination of peptide mass, peptide-ion sequence, and collision-cross section of MALDI-generated ions, providing information critical to the identification of unknown components in complex proteomic samples.

Original languageEnglish (US)
Pages (from-to)907-914
Number of pages8
JournalJournal of the American Society for Mass Spectrometry
Volume20
Issue number6
DOIs
StatePublished - Jun 2009
Externally publishedYes

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Mass spectrometry
Mass Spectrometry
Ions
Experiments
Peptides
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Vacuum
Pressure gradient
Kinetic energy
Proteomics
Ionization
Desorption
Lasers
Chemical activation
Electric fields
Pressure

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

Cite this

A Novel Approach to Collision-Induced Dissociation (CID) for Ion Mobility-Mass Spectrometry Experiments. / Becker, Christopher; Fernandez-Lima, Francisco A.; Gillig, Kent J.; Russell, William; Cologna, Stephanie M.; Russell, David H.

In: Journal of the American Society for Mass Spectrometry, Vol. 20, No. 6, 06.2009, p. 907-914.

Research output: Contribution to journalArticle

Becker, Christopher ; Fernandez-Lima, Francisco A. ; Gillig, Kent J. ; Russell, William ; Cologna, Stephanie M. ; Russell, David H. / A Novel Approach to Collision-Induced Dissociation (CID) for Ion Mobility-Mass Spectrometry Experiments. In: Journal of the American Society for Mass Spectrometry. 2009 ; Vol. 20, No. 6. pp. 907-914.
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