A novel conjugative plasmid from Enterococcus faecalis E99 enhances resistance to ultraviolet radiation

Phillip S. Coburn, Arto S. Baghdayan, Nikki Craig, Adam Burroughs, Preeti Tendolkar, Kris Miller, Fares Z. Najar, Bruce A. Roe, Nathan Shankar

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Enterococcus faecalis has emerged as a prominent healthcare-associated pathogen frequently encountered in bacteremia, endocarditis, urinary tract infection, and as a leading cause of antibiotic-resistant infections. We recently demonstrated a capacity for high-level biofilm formation by a clinical E. faecalis isolate, E99. This high biofilm-forming phenotype was attributable to a novel locus, designated bee, specifying a pilus at the bacterial cell surface and localized to a large ∼80. kb conjugative plasmid. To better understand the origin of the bee locus, as well as to potentially identify additional factors important to the biology and pathogenesis of strain E99, we sequenced the entire plasmid. The nucleotide sequence of the plasmid, designated pBEE99, revealed large regions of identity to the previously characterized conjugative plasmid pCF10. In addition to the bee locus, pBEE99 possesses an open reading frame potentially encoding aggregation substance, as well as open reading frames putatively encoding polypeptides with 60% to 99% identity at the amino acid level to proteins involved in regulation of the pheromone response and conjugal transfer of pCF10. However, strain E99 did not respond to the cCF10 pheromone in clumping assays. While pBEE99 was found to be devoid of any readily recognizable antibiotic resistance determinants, it carries two non-identical impB/. mucB/. samB-type genes, as well as genes potentially encoding a two-component bacteriocin similar to that encoded on pYI14. Although no bacteriocin activity was detected from an OG1RF transconjugant carrying pBEE99 against strain FA2-2, it was approximately an order of magnitude more resistant to ultraviolet radiation. Moreover, curing strain E99 of this plasmid significantly reduced its ability to survive UV exposure. Therefore, pBEE99 represents a novel conjugative plasmid that confers biofilm-forming and enhanced UV resistance traits that might potentially impact the virulence and/or fitness of E. faecalis.

Original languageEnglish (US)
Pages (from-to)18-25
Number of pages8
JournalPlasmid
Volume64
Issue number1
DOIs
StatePublished - Jul 2010
Externally publishedYes

Fingerprint

Enterococcus faecalis
Plasmids
Radiation
Bees
Biofilms
Bacteriocins
Pheromones
Open Reading Frames
Bacterial Fimbriae
Microbial Drug Resistance
Bacteremia
Endocarditis
Urinary Tract Infections
Genes
Virulence
Anti-Bacterial Agents
Delivery of Health Care
Phenotype
Amino Acids
Peptides

Keywords

  • Bacteriocin
  • Bee locus
  • Conjugation
  • Enterococcus faecalis
  • PBEE99
  • Ultraviolet radiation resistance

ASJC Scopus subject areas

  • Molecular Biology
  • Medicine(all)

Cite this

Coburn, P. S., Baghdayan, A. S., Craig, N., Burroughs, A., Tendolkar, P., Miller, K., ... Shankar, N. (2010). A novel conjugative plasmid from Enterococcus faecalis E99 enhances resistance to ultraviolet radiation. Plasmid, 64(1), 18-25. https://doi.org/10.1016/j.plasmid.2010.03.001

A novel conjugative plasmid from Enterococcus faecalis E99 enhances resistance to ultraviolet radiation. / Coburn, Phillip S.; Baghdayan, Arto S.; Craig, Nikki; Burroughs, Adam; Tendolkar, Preeti; Miller, Kris; Najar, Fares Z.; Roe, Bruce A.; Shankar, Nathan.

In: Plasmid, Vol. 64, No. 1, 07.2010, p. 18-25.

Research output: Contribution to journalArticle

Coburn, PS, Baghdayan, AS, Craig, N, Burroughs, A, Tendolkar, P, Miller, K, Najar, FZ, Roe, BA & Shankar, N 2010, 'A novel conjugative plasmid from Enterococcus faecalis E99 enhances resistance to ultraviolet radiation', Plasmid, vol. 64, no. 1, pp. 18-25. https://doi.org/10.1016/j.plasmid.2010.03.001
Coburn, Phillip S. ; Baghdayan, Arto S. ; Craig, Nikki ; Burroughs, Adam ; Tendolkar, Preeti ; Miller, Kris ; Najar, Fares Z. ; Roe, Bruce A. ; Shankar, Nathan. / A novel conjugative plasmid from Enterococcus faecalis E99 enhances resistance to ultraviolet radiation. In: Plasmid. 2010 ; Vol. 64, No. 1. pp. 18-25.
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