A novel defective recombinant porcine enterovirus G virus carrying a porcine torovirus papain-like cysteine protease gene and a putative anti-apoptosis gene in place of viral structural protein genes

Ryo Imai, Makoto Nagai, Mami Oba, Shoichi Sakaguchi, Makoto Ujike, Ruka Kimura, M. Kida, Tsuneyuki Masuda, M. Kuroda, Rongduo Wen, Kaixin Li, Y. Katayama, Yuki Naoi, Shinobu Tsuchiaka, Tsutomu Omatsu, Hiroshi Yamazato, Shinji Makino, Tetsuya Mizutani

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Enterovirus G (EV-G) belongs to the family of Picornaviridae. Two types of recombinant porcine EV-Gs carrying papain-like cysteine protease (PLCP) gene of porcine torovirus, a virus in Coronaviridae, are reported. Type 1 recombinant EV-Gs are detected in pig feces in Japan, USA, and Belgium and carry the PLPC gene at the junction site of 2C/3A genes, while PLPC gene replaces the viral structural genes in type 2 recombinant EV-G detected in pig feces in a Chinese farm. We identified a novel type 2 recombinant EV-G carrying the PLCP gene with flanking sequences in place of the viral structural genes in pig feces in Japan. The ~0.3 kb-long upstream flanking sequence had no sequence homology with any proteins deposited in GenBank, while the downstream ~0.9 kb-long flanking sequence included a domain having high amino acid sequence homology with a baculoviral inhibitor of apoptosis repeat superfamily. The pig feces, where the novel type 2 recombinant EV-G was detected, also carried type 1 recombinant EV-G. The amount of type 1 and type 2 recombinant EV-G genomes was almost same in the pig feces. Although the phylogenetic analysis suggested that these two recombinant EV-Gs have independently evolved, type 1 recombinant EV-G might have served as a helper virus by providing viral structural proteins for dissemination of the type 2 recombinant EV-G.

Original languageEnglish (US)
Article number103975
JournalInfection, Genetics and Evolution
Volume75
DOIs
StatePublished - Nov 2019

Fingerprint

Enterovirus G
Porcine torovirus
Porcine Enteroviruses
Torovirus
Porcine enterovirus
Viral Structural Proteins
Papain
Cysteine Proteases
papain
Enterovirus
cysteine proteinases
viral proteins
structural proteins
apoptosis
virus
Feces
Swine
Apoptosis
pig
feces

Keywords

  • Enterovirus
  • Recombination
  • Torovirus

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

Cite this

A novel defective recombinant porcine enterovirus G virus carrying a porcine torovirus papain-like cysteine protease gene and a putative anti-apoptosis gene in place of viral structural protein genes. / Imai, Ryo; Nagai, Makoto; Oba, Mami; Sakaguchi, Shoichi; Ujike, Makoto; Kimura, Ruka; Kida, M.; Masuda, Tsuneyuki; Kuroda, M.; Wen, Rongduo; Li, Kaixin; Katayama, Y.; Naoi, Yuki; Tsuchiaka, Shinobu; Omatsu, Tsutomu; Yamazato, Hiroshi; Makino, Shinji; Mizutani, Tetsuya.

In: Infection, Genetics and Evolution, Vol. 75, 103975, 11.2019.

Research output: Contribution to journalArticle

Imai, R, Nagai, M, Oba, M, Sakaguchi, S, Ujike, M, Kimura, R, Kida, M, Masuda, T, Kuroda, M, Wen, R, Li, K, Katayama, Y, Naoi, Y, Tsuchiaka, S, Omatsu, T, Yamazato, H, Makino, S & Mizutani, T 2019, 'A novel defective recombinant porcine enterovirus G virus carrying a porcine torovirus papain-like cysteine protease gene and a putative anti-apoptosis gene in place of viral structural protein genes', Infection, Genetics and Evolution, vol. 75, 103975. https://doi.org/10.1016/j.meegid.2019.103975
Imai, Ryo ; Nagai, Makoto ; Oba, Mami ; Sakaguchi, Shoichi ; Ujike, Makoto ; Kimura, Ruka ; Kida, M. ; Masuda, Tsuneyuki ; Kuroda, M. ; Wen, Rongduo ; Li, Kaixin ; Katayama, Y. ; Naoi, Yuki ; Tsuchiaka, Shinobu ; Omatsu, Tsutomu ; Yamazato, Hiroshi ; Makino, Shinji ; Mizutani, Tetsuya. / A novel defective recombinant porcine enterovirus G virus carrying a porcine torovirus papain-like cysteine protease gene and a putative anti-apoptosis gene in place of viral structural protein genes. In: Infection, Genetics and Evolution. 2019 ; Vol. 75.
@article{efb6e730dd6740ba96177cfb39de0d35,
title = "A novel defective recombinant porcine enterovirus G virus carrying a porcine torovirus papain-like cysteine protease gene and a putative anti-apoptosis gene in place of viral structural protein genes",
abstract = "Enterovirus G (EV-G) belongs to the family of Picornaviridae. Two types of recombinant porcine EV-Gs carrying papain-like cysteine protease (PLCP) gene of porcine torovirus, a virus in Coronaviridae, are reported. Type 1 recombinant EV-Gs are detected in pig feces in Japan, USA, and Belgium and carry the PLPC gene at the junction site of 2C/3A genes, while PLPC gene replaces the viral structural genes in type 2 recombinant EV-G detected in pig feces in a Chinese farm. We identified a novel type 2 recombinant EV-G carrying the PLCP gene with flanking sequences in place of the viral structural genes in pig feces in Japan. The ~0.3 kb-long upstream flanking sequence had no sequence homology with any proteins deposited in GenBank, while the downstream ~0.9 kb-long flanking sequence included a domain having high amino acid sequence homology with a baculoviral inhibitor of apoptosis repeat superfamily. The pig feces, where the novel type 2 recombinant EV-G was detected, also carried type 1 recombinant EV-G. The amount of type 1 and type 2 recombinant EV-G genomes was almost same in the pig feces. Although the phylogenetic analysis suggested that these two recombinant EV-Gs have independently evolved, type 1 recombinant EV-G might have served as a helper virus by providing viral structural proteins for dissemination of the type 2 recombinant EV-G.",
keywords = "Enterovirus, Recombination, Torovirus",
author = "Ryo Imai and Makoto Nagai and Mami Oba and Shoichi Sakaguchi and Makoto Ujike and Ruka Kimura and M. Kida and Tsuneyuki Masuda and M. Kuroda and Rongduo Wen and Kaixin Li and Y. Katayama and Yuki Naoi and Shinobu Tsuchiaka and Tsutomu Omatsu and Hiroshi Yamazato and Shinji Makino and Tetsuya Mizutani",
year = "2019",
month = "11",
doi = "10.1016/j.meegid.2019.103975",
language = "English (US)",
volume = "75",
journal = "Infection, Genetics and Evolution",
issn = "1567-1348",
publisher = "Elsevier",

}

TY - JOUR

T1 - A novel defective recombinant porcine enterovirus G virus carrying a porcine torovirus papain-like cysteine protease gene and a putative anti-apoptosis gene in place of viral structural protein genes

AU - Imai, Ryo

AU - Nagai, Makoto

AU - Oba, Mami

AU - Sakaguchi, Shoichi

AU - Ujike, Makoto

AU - Kimura, Ruka

AU - Kida, M.

AU - Masuda, Tsuneyuki

AU - Kuroda, M.

AU - Wen, Rongduo

AU - Li, Kaixin

AU - Katayama, Y.

AU - Naoi, Yuki

AU - Tsuchiaka, Shinobu

AU - Omatsu, Tsutomu

AU - Yamazato, Hiroshi

AU - Makino, Shinji

AU - Mizutani, Tetsuya

PY - 2019/11

Y1 - 2019/11

N2 - Enterovirus G (EV-G) belongs to the family of Picornaviridae. Two types of recombinant porcine EV-Gs carrying papain-like cysteine protease (PLCP) gene of porcine torovirus, a virus in Coronaviridae, are reported. Type 1 recombinant EV-Gs are detected in pig feces in Japan, USA, and Belgium and carry the PLPC gene at the junction site of 2C/3A genes, while PLPC gene replaces the viral structural genes in type 2 recombinant EV-G detected in pig feces in a Chinese farm. We identified a novel type 2 recombinant EV-G carrying the PLCP gene with flanking sequences in place of the viral structural genes in pig feces in Japan. The ~0.3 kb-long upstream flanking sequence had no sequence homology with any proteins deposited in GenBank, while the downstream ~0.9 kb-long flanking sequence included a domain having high amino acid sequence homology with a baculoviral inhibitor of apoptosis repeat superfamily. The pig feces, where the novel type 2 recombinant EV-G was detected, also carried type 1 recombinant EV-G. The amount of type 1 and type 2 recombinant EV-G genomes was almost same in the pig feces. Although the phylogenetic analysis suggested that these two recombinant EV-Gs have independently evolved, type 1 recombinant EV-G might have served as a helper virus by providing viral structural proteins for dissemination of the type 2 recombinant EV-G.

AB - Enterovirus G (EV-G) belongs to the family of Picornaviridae. Two types of recombinant porcine EV-Gs carrying papain-like cysteine protease (PLCP) gene of porcine torovirus, a virus in Coronaviridae, are reported. Type 1 recombinant EV-Gs are detected in pig feces in Japan, USA, and Belgium and carry the PLPC gene at the junction site of 2C/3A genes, while PLPC gene replaces the viral structural genes in type 2 recombinant EV-G detected in pig feces in a Chinese farm. We identified a novel type 2 recombinant EV-G carrying the PLCP gene with flanking sequences in place of the viral structural genes in pig feces in Japan. The ~0.3 kb-long upstream flanking sequence had no sequence homology with any proteins deposited in GenBank, while the downstream ~0.9 kb-long flanking sequence included a domain having high amino acid sequence homology with a baculoviral inhibitor of apoptosis repeat superfamily. The pig feces, where the novel type 2 recombinant EV-G was detected, also carried type 1 recombinant EV-G. The amount of type 1 and type 2 recombinant EV-G genomes was almost same in the pig feces. Although the phylogenetic analysis suggested that these two recombinant EV-Gs have independently evolved, type 1 recombinant EV-G might have served as a helper virus by providing viral structural proteins for dissemination of the type 2 recombinant EV-G.

KW - Enterovirus

KW - Recombination

KW - Torovirus

UR - http://www.scopus.com/inward/record.url?scp=85073650283&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85073650283&partnerID=8YFLogxK

U2 - 10.1016/j.meegid.2019.103975

DO - 10.1016/j.meegid.2019.103975

M3 - Article

C2 - 31344488

AN - SCOPUS:85073650283

VL - 75

JO - Infection, Genetics and Evolution

JF - Infection, Genetics and Evolution

SN - 1567-1348

M1 - 103975

ER -