A novel dinitrophenylglutathione-stimulated ATPase is present in human erythrocyte membranes

Edward F. LaBelle, Shivendra V. Singh, Hassan Ahmad, Leszek Wronski, Satish Srivastava, Yogesh C. Awasthi

Research output: Contribution to journalArticle

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Abstract

Vesicles prepared from human erythrocyte membranes were found to catalyze ATP hydrolysis that was stimulated by dinitrophenylglutathione (Dnp-SG). This activity was dependent on temperature and Me2+ and independent of ion pump ATPases present in erythrocyte membranes. The activity was a linear function of protein and time up to 60 min. The Km values of ATPase for Dnp-SG and ATP were found to be 49 μM and 1.67 mM, respectively. This suggests that in erythrocytes, the transport of Dnp-SG requires direct enzymatic hydrolysis of ATP and both Dnp-SG-stimulated ATPase activity and the ATP-dependent efflux of Dnp-SG from erythrocytes represent different activities of the same protein.

Original languageEnglish (US)
Pages (from-to)53-56
Number of pages4
JournalFEBS Letters
Volume228
Issue number1
DOIs
StatePublished - Feb 8 1988

Fingerprint

Erythrocyte Membrane
Adenosine Triphosphatases
Adenosine Triphosphate
Membranes
Hydrolysis
Erythrocytes
Ion Pumps
Enzymatic hydrolysis
Proteins
Temperature
S-(2,4-dinitrophenyl)glutathione
S-(dinitrophenyl)glutathione ATPase

Keywords

  • Dinitrophenylglutathione
  • Erythrocyte
  • Membrane
  • Transport

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

LaBelle, E. F., Singh, S. V., Ahmad, H., Wronski, L., Srivastava, S., & Awasthi, Y. C. (1988). A novel dinitrophenylglutathione-stimulated ATPase is present in human erythrocyte membranes. FEBS Letters, 228(1), 53-56. https://doi.org/10.1016/0014-5793(88)80583-0

A novel dinitrophenylglutathione-stimulated ATPase is present in human erythrocyte membranes. / LaBelle, Edward F.; Singh, Shivendra V.; Ahmad, Hassan; Wronski, Leszek; Srivastava, Satish; Awasthi, Yogesh C.

In: FEBS Letters, Vol. 228, No. 1, 08.02.1988, p. 53-56.

Research output: Contribution to journalArticle

LaBelle, EF, Singh, SV, Ahmad, H, Wronski, L, Srivastava, S & Awasthi, YC 1988, 'A novel dinitrophenylglutathione-stimulated ATPase is present in human erythrocyte membranes', FEBS Letters, vol. 228, no. 1, pp. 53-56. https://doi.org/10.1016/0014-5793(88)80583-0
LaBelle, Edward F. ; Singh, Shivendra V. ; Ahmad, Hassan ; Wronski, Leszek ; Srivastava, Satish ; Awasthi, Yogesh C. / A novel dinitrophenylglutathione-stimulated ATPase is present in human erythrocyte membranes. In: FEBS Letters. 1988 ; Vol. 228, No. 1. pp. 53-56.
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