A novel Ehrlichia genotype strain distinguished by the TRP36 gene naturally infects cattle in Brazil and causes clinical manifestations associated with ehrlichiosis

Daniel M. Aguiar, Thayza F. Ziliani, Xiaofeng Zhang, Andreia L T Melo, Ísis A. Braga, Rute Witter, Leodil C. Freitas, André L H Rondelli, Michele A. Luis, Eveline C B Sorte, Felipe W. Jaune, Vamilton A. Santarém, Mauricio C. Horta, Carolina A. Pescador, Edson M. Colodel, Herbert S. Soares, Richard C. Pacheco, Selma S M Onuma, Marcelo B. Labruna, Jere McBride

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

A novel Ehrlichia genotype most closely related to E. canis was reported in North American cattle in 2010, and a similar agent was subsequently identified in the hemolymph of Brazilian Rhipicephalus (Boophilus) microplus ticks and isolated in 2012. The purpose of this study was to determine whether this or other novel ehrlichial agents naturally infect Brazilian cattle. Using PCR targeting the genus-conserved dsb gene, DNA from this novel ehrlichial agent in Brazilian cattle was detected. Attempts to isolate the organism in vitro were performed using DH82 cells, but morulae and ehrlichial DNA could only be detected for approximately one month. In order to further molecularly characterize the organism, PCR was performed using primers specific for multiple E. canis genes (dsb, rrs, and trp36). Sequence obtained from the conserved rrs and dsb genes demonstrated that the organism was 99-100% identical to the novel Ehrlichia genotypes previously reported in North American cattle (rrs gene) and Brazilian ticks (rrs and dsb genes). However, analysis of the trp36 gene revealed substantial strain diversity between these Ehrlichia genotypes strains, including divergent tandem repeat sequences. In order to obtain preliminary information on the potential pathogenicity of this ehrlichial agent and clinical course of infection, a calf was experimentally infected. The calf showed clinical signs of ehrlichiosis, including fever, depression, lethargy, thrombocytopenia, and morulae were observed in peripheral blood monocytes. This study reports a previously unrecognized disease-causing Ehrlichia sp. in Brazilian cattle that is consistent with the genotype previously described in North America cattle and ticks from Brazil. Hence, it is likely that this is the organism previously identified as Ehrlichia bovis in Brazil in 1982. Furthermore, we have concluded that strains of these Ehrlichia genotypes can be molecularly distinguished by the trp36 gene, which has been widely utilized to define E. canis strain diversity.

Original languageEnglish (US)
Pages (from-to)537-544
Number of pages8
JournalTicks and Tick-borne Diseases
Volume5
Issue number5
DOIs
StatePublished - 2014

Fingerprint

Ehrlichia
Ehrlichiosis
ehrlichiosis
Brazil
Genotype
genotype
cattle
Canis
Ticks
Genes
genes
ticks
Morula
strain differences
morula
organisms
Anaplasma bovis
calves
Rhipicephalus
Polymerase Chain Reaction

Keywords

  • A. bovis
  • Bovine
  • Brazil
  • Dsb
  • E. bovis
  • E. canis

ASJC Scopus subject areas

  • Infectious Diseases
  • Insect Science
  • Parasitology
  • Microbiology
  • Medicine(all)

Cite this

A novel Ehrlichia genotype strain distinguished by the TRP36 gene naturally infects cattle in Brazil and causes clinical manifestations associated with ehrlichiosis. / Aguiar, Daniel M.; Ziliani, Thayza F.; Zhang, Xiaofeng; Melo, Andreia L T; Braga, Ísis A.; Witter, Rute; Freitas, Leodil C.; Rondelli, André L H; Luis, Michele A.; Sorte, Eveline C B; Jaune, Felipe W.; Santarém, Vamilton A.; Horta, Mauricio C.; Pescador, Carolina A.; Colodel, Edson M.; Soares, Herbert S.; Pacheco, Richard C.; Onuma, Selma S M; Labruna, Marcelo B.; McBride, Jere.

In: Ticks and Tick-borne Diseases, Vol. 5, No. 5, 2014, p. 537-544.

Research output: Contribution to journalArticle

Aguiar, DM, Ziliani, TF, Zhang, X, Melo, ALT, Braga, ÍA, Witter, R, Freitas, LC, Rondelli, ALH, Luis, MA, Sorte, ECB, Jaune, FW, Santarém, VA, Horta, MC, Pescador, CA, Colodel, EM, Soares, HS, Pacheco, RC, Onuma, SSM, Labruna, MB & McBride, J 2014, 'A novel Ehrlichia genotype strain distinguished by the TRP36 gene naturally infects cattle in Brazil and causes clinical manifestations associated with ehrlichiosis', Ticks and Tick-borne Diseases, vol. 5, no. 5, pp. 537-544. https://doi.org/10.1016/j.ttbdis.2014.03.010
Aguiar, Daniel M. ; Ziliani, Thayza F. ; Zhang, Xiaofeng ; Melo, Andreia L T ; Braga, Ísis A. ; Witter, Rute ; Freitas, Leodil C. ; Rondelli, André L H ; Luis, Michele A. ; Sorte, Eveline C B ; Jaune, Felipe W. ; Santarém, Vamilton A. ; Horta, Mauricio C. ; Pescador, Carolina A. ; Colodel, Edson M. ; Soares, Herbert S. ; Pacheco, Richard C. ; Onuma, Selma S M ; Labruna, Marcelo B. ; McBride, Jere. / A novel Ehrlichia genotype strain distinguished by the TRP36 gene naturally infects cattle in Brazil and causes clinical manifestations associated with ehrlichiosis. In: Ticks and Tick-borne Diseases. 2014 ; Vol. 5, No. 5. pp. 537-544.
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AU - Aguiar, Daniel M.

AU - Ziliani, Thayza F.

AU - Zhang, Xiaofeng

AU - Melo, Andreia L T

AU - Braga, Ísis A.

AU - Witter, Rute

AU - Freitas, Leodil C.

AU - Rondelli, André L H

AU - Luis, Michele A.

AU - Sorte, Eveline C B

AU - Jaune, Felipe W.

AU - Santarém, Vamilton A.

AU - Horta, Mauricio C.

AU - Pescador, Carolina A.

AU - Colodel, Edson M.

AU - Soares, Herbert S.

AU - Pacheco, Richard C.

AU - Onuma, Selma S M

AU - Labruna, Marcelo B.

AU - McBride, Jere

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