A novel motility effect of tachykinins in normal and inflamed colon

Mikio Tsukamoto, Sushil K. Sarna, Robert E. Condon

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The role of tachykinins in stimulating phasic and giant migrating contractions (GMCs) in the normal and inflamed colon in conscious dogs was investigated by close-intra-arterial infusions of test substances. At low doses (0.1 nmol), substance P and neurokinin (NK1) receptor agonist |[Sar9,Met(O2)11]substance P| stimulated phasic contractions only. At higher doses (2.0 nmol), they stimulated phasic contractions and GMCs. The phasic contractions were blocked partially but significantly by prior close- intra-arterial infusions of tetrodotoxin and atropine but not by hexamethonium. NK1 receptor antagonist partially but significantly inhibited the phasic contractile response to substance P, whereas NK2 and NK3 receptor antagonists had no significant effect. The contractile response to NK2 receptor agonist was less than one-half of the response to substance P; NK3 receptor agonist did not stimulate any contractile activity. The stimulation of GMCs by higher doses of substance P was not blocked by prior infusions of atropine, tetrodotoxin, or NK1, NK2, and NK3 receptor antagonists, nor was the contractile response to substance P blocked by H1 and H2 receptor antagonists. Inflammation depressed the phasic contractile response but enhanced the stimulation of GMCs by substance P. The ability of substance P to stimulate GMCs is novel and suggests its potential role in increasing the frequency of these contractions during colonic inflammation.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume272
Issue number6 35-6
StatePublished - Jun 1997
Externally publishedYes

Fingerprint

Tachykinins
Substance P
Colon
Intra Arterial Infusions
Tetrodotoxin
Atropine
Inflammation
Neurokinin-1 Receptors
Histamine H1 Receptors
Hexamethonium
Histamine H2 Receptors
Dogs

Keywords

  • Giant migrating contractions
  • Histamine
  • Inflammation
  • Mast cells
  • Neurokinin receptors
  • Substance P

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology
  • Physiology (medical)

Cite this

A novel motility effect of tachykinins in normal and inflamed colon. / Tsukamoto, Mikio; Sarna, Sushil K.; Condon, Robert E.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 272, No. 6 35-6, 06.1997.

Research output: Contribution to journalArticle

Tsukamoto, Mikio ; Sarna, Sushil K. ; Condon, Robert E. / A novel motility effect of tachykinins in normal and inflamed colon. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 1997 ; Vol. 272, No. 6 35-6.
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