A novel MSC-seeded triphasic construct for the repair of osteochondral defects

B. Marquass, Jeremy Somerson, P. Hepp, T. Aigner, S. Schwan, A. Bader, C. Josten, M. Zscharnack, R. M. Schulz

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSC) are increasingly replacing chondrocytes in tissue engineering based research for treatment of osteochondral defects. The aim of this work was to determine whether repair of critical-size chronic osteochondral defects in an ovine model using MSC-seeded triphasic constructs would show results comparable to osteochondral autografting (OATS). Triphasic implants were engineered using a beta-tricalcium phosphate osseous phase, an intermediate activated plasma phase, and a collagen I hydrogel chondral phase. Autologous MSCs were used to seed the implants, with chondrogenic predifferentiation of the cells used in the cartilage phase. Osteochondral defects of 4.0 mm diameter were created bilaterally in ovine knees (n = 10). Six weeks later, half of the lesions were treated with OATS and half with triphasic constructs. The knees were dissected at 6 or 12 months. With the chosen study design we were not able to demonstrate significant differences between the histological scores of both groups. Subcategory analysis of O'Driscoll scores showed superior cartilage bonding in the 6-month triphasic group compared to the autograft group. The 12-month autograft group showed superior cartilage matrix morphology compared to the 12-month triphasic group. Macroscopic and biomechanical analysis showed no significant differences at 12 months. Autologous MSC-seeded triphasic implants showed comparable repair quality to osteochondral autografts in terms of histology and biomechanical testing.

Original languageEnglish (US)
Pages (from-to)1586-1599
Number of pages14
JournalJournal of Orthopaedic Research
Volume28
Issue number12
DOIs
StatePublished - Dec 2010
Externally publishedYes

Fingerprint

Mesenchymal Stromal Cells
Cartilage
Autografts
Knee
Sheep
Autologous Transplantation
Hydrogel
Tissue Engineering
Chondrocytes
Seeds
Histology
Collagen
Research
Therapeutics

Keywords

  • animal model
  • cartilage
  • mesenchymal stem cells
  • osteoarthritis
  • osteochondral autografting

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Medicine(all)

Cite this

Marquass, B., Somerson, J., Hepp, P., Aigner, T., Schwan, S., Bader, A., ... Schulz, R. M. (2010). A novel MSC-seeded triphasic construct for the repair of osteochondral defects. Journal of Orthopaedic Research, 28(12), 1586-1599. https://doi.org/10.1002/jor.21173

A novel MSC-seeded triphasic construct for the repair of osteochondral defects. / Marquass, B.; Somerson, Jeremy; Hepp, P.; Aigner, T.; Schwan, S.; Bader, A.; Josten, C.; Zscharnack, M.; Schulz, R. M.

In: Journal of Orthopaedic Research, Vol. 28, No. 12, 12.2010, p. 1586-1599.

Research output: Contribution to journalArticle

Marquass, B, Somerson, J, Hepp, P, Aigner, T, Schwan, S, Bader, A, Josten, C, Zscharnack, M & Schulz, RM 2010, 'A novel MSC-seeded triphasic construct for the repair of osteochondral defects', Journal of Orthopaedic Research, vol. 28, no. 12, pp. 1586-1599. https://doi.org/10.1002/jor.21173
Marquass, B. ; Somerson, Jeremy ; Hepp, P. ; Aigner, T. ; Schwan, S. ; Bader, A. ; Josten, C. ; Zscharnack, M. ; Schulz, R. M. / A novel MSC-seeded triphasic construct for the repair of osteochondral defects. In: Journal of Orthopaedic Research. 2010 ; Vol. 28, No. 12. pp. 1586-1599.
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