A novel, rapid assay for detection and differentiation of serotype-specific antibodies to Venezuelan equine encephalitis complex alphaviruses

Eryu Wang, Slobodan Paessler, Patricia Aguilar, Darci R. Smith, Lark L. Coffey, Wenli Kang, Martin Pfeffer, James Olson, Patrick J. Blair, Carolina Guevara, Jose Estrada-Franco, Scott Weaver

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15 Scopus citations

Abstract

An epitope-blocking enzyme-linked immunosorbent assay was developed for the rapid differentiation of serologic responses to enzootic variety IE and ID versus epizootic variety IAB and IC strains of Venezuelan equine encephalitis (VEE) virus. Two monoclonal antibodies that differentially recognize epizootic versus enzootic VEE virus epitopes were used to measure the serotype-specific blocking abilities of antibodies in sera of naturally infected humans, equines, and bovines, as well as in experimentally infected equines. The assay is simple, species-independent, rapid, and sensitive, and will improve surveillance for VEE emergence. It could also be used to determine the epidemic potential of a VEE virus following an intentional introduction for bioterrorism.

Original languageEnglish (US)
Pages (from-to)805-810
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume72
Issue number6
StatePublished - Jun 2005

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ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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