A novel reporter system for neutralizing and enhancing antibody assay against dengue virus

Ke Yu Song, Hui Zhao, Zhen You Jiang, Xiao Feng Li, Yong Qiang Deng, Tao Jiang, Shun Ya Zhu, Pei Yong Shi, Bo Zhang, Fu Chun Zhang, E. De Qin, Cheng Feng Qin

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Abstract. Background: Dengue virus (DENV) still poses a global public health threat, and no vaccine or antiviral therapy is currently available. Antibody plays distinct roles in controlling DENV infections. Neutralizing antibody is protective against DENV infection, whereas sub-neutralizing concentration of antibody can increase DENV infection, termed antibody-dependent enhancement (ADE). Plaque-based assay represents the most widely accepted method measuring neutralizing or enhancing antibodies. Results: In this study, a novel reporter virus-based system was developed for measuring neutralization and ADE activity. A stable Renilla luciferase reporter DENV (Luc-DENV) that can produce robust luciferase signals in BHK-21 and K562 cells were used to establish the assay and validated against traditional plaque-based assay. Luciferase value analysis using various known DENV-specific monoclonal antibodies showed good repeatability and a well linear correlation with conventional plaque-based assays. The newly developed assay was finally validated with clinical samples from infected animals and individuals. Conclusions: This reporter virus-based assay for neutralizing and enhancing antibody evaluation is rapid, lower cost, and high throughput, and will be helpful for laboratory detection and epidemiological investigation for DENV antibodies.

Original languageEnglish (US)
Article number44
JournalBMC Microbiology
Volume14
Issue number1
DOIs
StatePublished - Feb 18 2014
Externally publishedYes

Keywords

  • Dengue virus
  • Enhancing antibody
  • Luciferase assay
  • Neutralizing antibody

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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  • Cite this

    Song, K. Y., Zhao, H., Jiang, Z. Y., Li, X. F., Deng, Y. Q., Jiang, T., Zhu, S. Y., Shi, P. Y., Zhang, B., Zhang, F. C., Qin, E. D., & Qin, C. F. (2014). A novel reporter system for neutralizing and enhancing antibody assay against dengue virus. BMC Microbiology, 14(1), [44]. https://doi.org/10.1186/1471-2180-14-44