A novel reporter system for neutralizing and enhancing antibody assay against dengue virus

Ke Yu Song, Hui Zhao, Zhen You Jiang, Xiao Feng Li, Yong Qiang Deng, Tao Jiang, Shun Ya Zhu, Pei-Yong Shi, Bo Zhang, Fu Chun Zhang, E. De Qin, Cheng Feng Qin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Abstract. Background: Dengue virus (DENV) still poses a global public health threat, and no vaccine or antiviral therapy is currently available. Antibody plays distinct roles in controlling DENV infections. Neutralizing antibody is protective against DENV infection, whereas sub-neutralizing concentration of antibody can increase DENV infection, termed antibody-dependent enhancement (ADE). Plaque-based assay represents the most widely accepted method measuring neutralizing or enhancing antibodies. Results: In this study, a novel reporter virus-based system was developed for measuring neutralization and ADE activity. A stable Renilla luciferase reporter DENV (Luc-DENV) that can produce robust luciferase signals in BHK-21 and K562 cells were used to establish the assay and validated against traditional plaque-based assay. Luciferase value analysis using various known DENV-specific monoclonal antibodies showed good repeatability and a well linear correlation with conventional plaque-based assays. The newly developed assay was finally validated with clinical samples from infected animals and individuals. Conclusions: This reporter virus-based assay for neutralizing and enhancing antibody evaluation is rapid, lower cost, and high throughput, and will be helpful for laboratory detection and epidemiological investigation for DENV antibodies.

Original languageEnglish (US)
Article number44
JournalBMC Microbiology
Volume14
Issue number1
DOIs
StatePublished - Feb 18 2014
Externally publishedYes

Fingerprint

Dengue Virus
Blocking Antibodies
Neutralizing Antibodies
Antibody-Dependent Enhancement
Virus Diseases
Luciferases
Renilla Luciferases
Viruses
K562 Cells
Antibodies
Antiviral Agents
Vaccines
Public Health
Monoclonal Antibodies
Costs and Cost Analysis

Keywords

  • Dengue virus
  • Enhancing antibody
  • Luciferase assay
  • Neutralizing antibody

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Song, K. Y., Zhao, H., Jiang, Z. Y., Li, X. F., Deng, Y. Q., Jiang, T., ... Qin, C. F. (2014). A novel reporter system for neutralizing and enhancing antibody assay against dengue virus. BMC Microbiology, 14(1), [44]. https://doi.org/10.1186/1471-2180-14-44

A novel reporter system for neutralizing and enhancing antibody assay against dengue virus. / Song, Ke Yu; Zhao, Hui; Jiang, Zhen You; Li, Xiao Feng; Deng, Yong Qiang; Jiang, Tao; Zhu, Shun Ya; Shi, Pei-Yong; Zhang, Bo; Zhang, Fu Chun; Qin, E. De; Qin, Cheng Feng.

In: BMC Microbiology, Vol. 14, No. 1, 44, 18.02.2014.

Research output: Contribution to journalArticle

Song, KY, Zhao, H, Jiang, ZY, Li, XF, Deng, YQ, Jiang, T, Zhu, SY, Shi, P-Y, Zhang, B, Zhang, FC, Qin, ED & Qin, CF 2014, 'A novel reporter system for neutralizing and enhancing antibody assay against dengue virus', BMC Microbiology, vol. 14, no. 1, 44. https://doi.org/10.1186/1471-2180-14-44
Song, Ke Yu ; Zhao, Hui ; Jiang, Zhen You ; Li, Xiao Feng ; Deng, Yong Qiang ; Jiang, Tao ; Zhu, Shun Ya ; Shi, Pei-Yong ; Zhang, Bo ; Zhang, Fu Chun ; Qin, E. De ; Qin, Cheng Feng. / A novel reporter system for neutralizing and enhancing antibody assay against dengue virus. In: BMC Microbiology. 2014 ; Vol. 14, No. 1.
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