A Novel Rhabdovirus Associated with Acute Hemorrhagic Fever in Central Africa

Gilda Grard, Joseph N. Fair, Deanna Lee, Elizabeth Slikas, Imke Steffen, Jean Jacques Muyembe, Taylor Sittler, Narayanan Veeraraghavan, J. Graham Ruby, Chunlin Wang, Maria Makuwa, Prime Mulembakani, Robert B. Tesh, Jonna Mazet, Anne W. Rimoin, Travis Taylor, Bradley S. Schneider, Graham Simmons, Eric Delwart, Nathan D. WolfeCharles Y. Chiu, Eric M. Leroy

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

Deep sequencing was used to discover a novel rhabdovirus (Bas-Congo virus, or BASV) associated with a 2009 outbreak of 3 human cases of acute hemorrhagic fever in Mangala village, Democratic Republic of Congo (DRC), Africa. The cases, presenting over a 3-week period, were characterized by abrupt disease onset, high fever, mucosal hemorrhage, and, in two patients, death within 3 days. BASV was detected in an acute serum sample from the lone survivor at a concentration of 1.09×106 RNA copies/mL, and 98.2% of the genome was subsequently de novo assembled from ~140 million sequence reads. Phylogenetic analysis revealed that BASV is highly divergent and shares less than 34% amino acid identity with any other rhabdovirus. High convalescent neutralizing antibody titers of >1:1000 were detected in the survivor and an asymptomatic nurse directly caring for him, both of whom were health care workers, suggesting the potential for human-to-human transmission of BASV. The natural animal reservoir host or arthropod vector and precise mode of transmission for the virus remain unclear. BASV is an emerging human pathogen associated with acute hemorrhagic fever in Africa.

Original languageEnglish (US)
Article numbere1002924
JournalPLoS Pathogens
Volume8
Issue number9
DOIs
StatePublished - Sep 2012

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Rhabdoviridae
Central Africa
Fever
Survivors
Crimean-Congo hemorrhagic fever virus
Arthropod Vectors
Democratic Republic of the Congo
High-Throughput Nucleotide Sequencing
Neutralizing Antibodies
Disease Outbreaks
Nurses
Genome
RNA
Hemorrhage
Viruses
Delivery of Health Care
Amino Acids
Serum

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Grard, G., Fair, J. N., Lee, D., Slikas, E., Steffen, I., Muyembe, J. J., ... Leroy, E. M. (2012). A Novel Rhabdovirus Associated with Acute Hemorrhagic Fever in Central Africa. PLoS Pathogens, 8(9), [e1002924]. https://doi.org/10.1371/journal.ppat.1002924

A Novel Rhabdovirus Associated with Acute Hemorrhagic Fever in Central Africa. / Grard, Gilda; Fair, Joseph N.; Lee, Deanna; Slikas, Elizabeth; Steffen, Imke; Muyembe, Jean Jacques; Sittler, Taylor; Veeraraghavan, Narayanan; Ruby, J. Graham; Wang, Chunlin; Makuwa, Maria; Mulembakani, Prime; Tesh, Robert B.; Mazet, Jonna; Rimoin, Anne W.; Taylor, Travis; Schneider, Bradley S.; Simmons, Graham; Delwart, Eric; Wolfe, Nathan D.; Chiu, Charles Y.; Leroy, Eric M.

In: PLoS Pathogens, Vol. 8, No. 9, e1002924, 09.2012.

Research output: Contribution to journalArticle

Grard, G, Fair, JN, Lee, D, Slikas, E, Steffen, I, Muyembe, JJ, Sittler, T, Veeraraghavan, N, Ruby, JG, Wang, C, Makuwa, M, Mulembakani, P, Tesh, RB, Mazet, J, Rimoin, AW, Taylor, T, Schneider, BS, Simmons, G, Delwart, E, Wolfe, ND, Chiu, CY & Leroy, EM 2012, 'A Novel Rhabdovirus Associated with Acute Hemorrhagic Fever in Central Africa', PLoS Pathogens, vol. 8, no. 9, e1002924. https://doi.org/10.1371/journal.ppat.1002924
Grard G, Fair JN, Lee D, Slikas E, Steffen I, Muyembe JJ et al. A Novel Rhabdovirus Associated with Acute Hemorrhagic Fever in Central Africa. PLoS Pathogens. 2012 Sep;8(9). e1002924. https://doi.org/10.1371/journal.ppat.1002924
Grard, Gilda ; Fair, Joseph N. ; Lee, Deanna ; Slikas, Elizabeth ; Steffen, Imke ; Muyembe, Jean Jacques ; Sittler, Taylor ; Veeraraghavan, Narayanan ; Ruby, J. Graham ; Wang, Chunlin ; Makuwa, Maria ; Mulembakani, Prime ; Tesh, Robert B. ; Mazet, Jonna ; Rimoin, Anne W. ; Taylor, Travis ; Schneider, Bradley S. ; Simmons, Graham ; Delwart, Eric ; Wolfe, Nathan D. ; Chiu, Charles Y. ; Leroy, Eric M. / A Novel Rhabdovirus Associated with Acute Hemorrhagic Fever in Central Africa. In: PLoS Pathogens. 2012 ; Vol. 8, No. 9.
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