@article{8b7af360a2e54961be97f2e4574b134c,
title = "A pan-coronavirus RT-PCR assay for rapid viral screening of animal, human, and environmental specimens",
abstract = "We examined a collection of 386 animal, 451 human, and 109 archived bioaerosol samples with a new pan-species coronavirus molecular assay. Thirty-eight (4.02%) of 946 specimens yielded evidence of human or animal coronaviruses. Our findings demonstrate the utility of employing the pan-CoV RT-PCR assay in detecting varied coronavirus among human, animal, and environmental specimens. This RT-PCR assay might be employed as a screening diagnostic for early detection of coronaviruses incursions or prepandemic coronavirus emergence in animal or human populations.",
keywords = "Coronaviruses, Infectious diseases, One health, RT-PCR, Zoonotic diseases",
author = "Xinye Wang and Leshan Xiu and Binder, {Raquel A.} and Toh, {Teck Hock} and Lee, {Jeffrey Soon Yit} and Jakie Ting and Than, {Son T.} and Wenhao Qi and Coleman, {Kristen K.} and David Perera and Maijuan Ma and Gray, {Gregory C.}",
note = "Funding Information: This study was supported by funding from the National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH) (R01AI108993-01A1 to G.C.G.), from Duke University's Clinical Translational Science Institute (G.C.G), from US Naval Medical Research Center-Asia [W911QY-14-C-0043, SC-2016-SABER-003-002, SC-2017-SABER-010-001] and from Professor Gray's discretionary funding. We thank many professionals who collected these archived human, animal, and environmental samples Their names are listed in several original reports [ 8–12]. We also thank Dr. Changjun Bao and Dr. Xian Qi of the Jiangsu Provincial Center for Disease Control and Prevention for their support in providing archived animal samples. Funding Information: This study was supported by funding from the National Institute of Allergy and Infectious Diseases , National Institutes of Health (NIH) ( R01AI108993-01A1 to G.C.G. ), from Duke University{\textquoteright}s Clinical Translational Science Institute (G.C.G) , from US Naval Medical Research Center -Asia [ W911QY-14-C-0043 , SC-2016-SABER-003-002 , SC-2017-SABER-010-001 ] and from Professor Gray's discretionary funding. We thank many professionals who collected these archived human, animal, and environmental samples Their names are listed in several original reports [ 8–12 ]. We also thank Dr. Changjun Bao and Dr. Xian Qi of the Jiangsu Provincial Center for Disease Control and Prevention for their support in providing archived animal samples. Publisher Copyright: {\textcopyright} 2021 The Authors",
year = "2021",
month = dec,
doi = "10.1016/j.onehlt.2021.100274",
language = "English (US)",
volume = "13",
journal = "One Health",
issn = "2352-7714",
publisher = "Elsevier BV",
}