A pan-coronavirus RT-PCR assay for rapid viral screening of animal, human, and environmental specimens

Xinye Wang; Leshan Xiu; Raquel A. Binder; Teck Hock Toh; Jeffrey Soon Yit Lee; Jakie Ting; Son T. Than; Wenhao Qi; Kristen K. Coleman; David Perera; Maijuan Ma; Gregory C. Gray

doi:10.1016/j.onehlt.2021.100274

A pan-coronavirus RT-PCR assay for rapid viral screening of animal, human, and environmental specimens

Xinye Wang
, Leshan Xiu
, Raquel A. Binder
, Teck Hock Toh
, Jeffrey Soon Yit Lee
, Jakie Ting
, Son T. Than
, Wenhao Qi
, Kristen K. Coleman
, David Perera
, Maijuan Ma
, Gregory C. Gray

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We examined a collection of 386 animal, 451 human, and 109 archived bioaerosol samples with a new pan-species coronavirus molecular assay. Thirty-eight (4.02%) of 946 specimens yielded evidence of human or animal coronaviruses. Our findings demonstrate the utility of employing the pan-CoV RT-PCR assay in detecting varied coronavirus among human, animal, and environmental specimens. This RT-PCR assay might be employed as a screening diagnostic for early detection of coronaviruses incursions or prepandemic coronavirus emergence in animal or human populations.

Original language	English (US)
Article number	100274
Journal	One Health
Volume	13
DOIs	https://doi.org/10.1016/j.onehlt.2021.100274
State	Published - Dec 2021
Externally published	Yes

Keywords

Coronaviruses
Infectious diseases
One health
RT-PCR
Zoonotic diseases

ASJC Scopus subject areas

Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/j.onehlt.2021.100274

Cite this

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title = "A pan-coronavirus RT-PCR assay for rapid viral screening of animal, human, and environmental specimens",

abstract = "We examined a collection of 386 animal, 451 human, and 109 archived bioaerosol samples with a new pan-species coronavirus molecular assay. Thirty-eight (4.02\%) of 946 specimens yielded evidence of human or animal coronaviruses. Our findings demonstrate the utility of employing the pan-CoV RT-PCR assay in detecting varied coronavirus among human, animal, and environmental specimens. This RT-PCR assay might be employed as a screening diagnostic for early detection of coronaviruses incursions or prepandemic coronavirus emergence in animal or human populations.",

keywords = "Coronaviruses, Infectious diseases, One health, RT-PCR, Zoonotic diseases",

author = "Xinye Wang and Leshan Xiu and Binder, \{Raquel A.\} and Toh, \{Teck Hock\} and Lee, \{Jeffrey Soon Yit\} and Jakie Ting and Than, \{Son T.\} and Wenhao Qi and Coleman, \{Kristen K.\} and David Perera and Maijuan Ma and Gray, \{Gregory C.\}",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors",

year = "2021",

month = dec,

doi = "10.1016/j.onehlt.2021.100274",

language = "English (US)",

volume = "13",

journal = "One Health",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - A pan-coronavirus RT-PCR assay for rapid viral screening of animal, human, and environmental specimens

AU - Wang, Xinye

AU - Xiu, Leshan

AU - Binder, Raquel A.

AU - Toh, Teck Hock

AU - Lee, Jeffrey Soon Yit

AU - Ting, Jakie

AU - Than, Son T.

AU - Qi, Wenhao

AU - Coleman, Kristen K.

AU - Perera, David

AU - Ma, Maijuan

AU - Gray, Gregory C.

PY - 2021/12

Y1 - 2021/12

N2 - We examined a collection of 386 animal, 451 human, and 109 archived bioaerosol samples with a new pan-species coronavirus molecular assay. Thirty-eight (4.02%) of 946 specimens yielded evidence of human or animal coronaviruses. Our findings demonstrate the utility of employing the pan-CoV RT-PCR assay in detecting varied coronavirus among human, animal, and environmental specimens. This RT-PCR assay might be employed as a screening diagnostic for early detection of coronaviruses incursions or prepandemic coronavirus emergence in animal or human populations.

AB - We examined a collection of 386 animal, 451 human, and 109 archived bioaerosol samples with a new pan-species coronavirus molecular assay. Thirty-eight (4.02%) of 946 specimens yielded evidence of human or animal coronaviruses. Our findings demonstrate the utility of employing the pan-CoV RT-PCR assay in detecting varied coronavirus among human, animal, and environmental specimens. This RT-PCR assay might be employed as a screening diagnostic for early detection of coronaviruses incursions or prepandemic coronavirus emergence in animal or human populations.

KW - Coronaviruses

KW - Infectious diseases

KW - One health

KW - RT-PCR

KW - Zoonotic diseases

UR - https://www.scopus.com/pages/publications/85108688361

UR - https://www.scopus.com/pages/publications/85108688361#tab=citedBy

U2 - 10.1016/j.onehlt.2021.100274

DO - 10.1016/j.onehlt.2021.100274

M3 - Article

C2 - 34124332

AN - SCOPUS:85108688361

VL - 13

JO - One Health

JF - One Health

M1 - 100274

ER -

A pan-coronavirus RT-PCR assay for rapid viral screening of animal, human, and environmental specimens

Abstract

Keywords

ASJC Scopus subject areas

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