A peripheral neuroimmune link

Glutamate agonists upregulate NMDA NR1 receptor mRNA and protein, vimentin, TNF-α, and RANTES in cultured human synoviocytes

Terry A. McNearney, Yinghong Ma, Yueping Chen, Giulio Taglialatela, Huaizhi Yin, Wen Ru Zhang, Karin N. Westlund

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Human primary and clonal synovial cells were incubated with glutamate receptor agonists to assess their modulating influence on glutamate receptors N-methyl-D-aspartate (NMDA) NR1 and NR2 and inflammatory cytokines to determine potential for paracrine or autocrine (neurocrine) upregulation of glutamate receptors, as has been shown for bone and chondrocytes. Clonal SW982 synoviocytes constitutively express vimentin, smooth muscle actin (SMA), and NMDA NR1 and NR2. Coincubation (6 h) with glutamate agonists NMDA (5 μM), and the NMDA NR1 glycine site activator (±)1-aminocyclopentane-cis-1,3- dicarboxylic acid (5 μM), significantly increases cellular mRNA and protein levels of glutamate receptors, as well as increasing vimentin, SMA, tumor necrosis factor-α, and RANTES (regulated on activation, normal T-cell expressed and secreted), assessed qualitatively and quantitatively with nucleotide amplification, image analysis of immunocytochemical staining, fluorescein-activated cell sorting, Western blotting, and immunoassays. Human primary synovial cells harvested from patients with arthritic conditions also constitutively expressed NMDA NR1 with increases after agonist treatment. Glutamate receptor agonist-induced increases were blocked by the noncompetitive glutamate antagonist MK-801 (8 μg/ml) and NR1 blocking antibody. Coincubation with glutamate agonists and phorbol 12-myristate 13-acetate, a protein kinase C activator, significantly enhanced mean levels of TNF-α and RANTES in SW982 cell supernatants compared with incubation with either agent alone. Increases were diminished with protein kinase inhibitor and NR1 blocking antibody. The functional activation of glutamate receptors on human synoviocytes establishes a neurogenic cell signaling link between neurotransmitter glutamate released from nerve terminals and target cells in the joint capsule. The influence of glutamate on subsequent release of cellular proinflammatory mediators in non-neural tissue for activation of downstream immune events supports a peripheral neuroimmune link in arthritis.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume298
Issue number3
DOIs
StatePublished - Mar 2010

Fingerprint

Excitatory Amino Acid Agonists
Glutamate Receptors
Vimentin
N-Methyl-D-Aspartate Receptors
N-Methylaspartate
Up-Regulation
T-Lymphocytes
Messenger RNA
Proteins
Blocking Antibodies
Arthritis
Smooth Muscle
Actins
Glutamic Acid
Joint Capsule
Excitatory Amino Acid Antagonists
Dizocilpine Maleate
Protein Kinase Inhibitors
Chondrocytes
Fluorescein

Keywords

  • Arthritis
  • Cytokines, protein kinase C
  • Glutamate receptor
  • Neurogenic inflammation
  • Pain

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

A peripheral neuroimmune link : Glutamate agonists upregulate NMDA NR1 receptor mRNA and protein, vimentin, TNF-α, and RANTES in cultured human synoviocytes. / McNearney, Terry A.; Ma, Yinghong; Chen, Yueping; Taglialatela, Giulio; Yin, Huaizhi; Zhang, Wen Ru; Westlund, Karin N.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 298, No. 3, 03.2010.

Research output: Contribution to journalArticle

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