A physico-chemical comparison of aortic receptors in rat hypertension models

Walter Meyer, Nancy R. Nichols, Hanh H. Nguyen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Rat models of genetic hypertension include spontaneous hypertension and resistance or sensitivity to mineralocorticoid and salt induced hypertension. Previously, altered aldosterone binding to corticoid receptor I was found in aortic smooth muscle cells cultured from Fischer 344 rats which are extremely resistant to steroid and salt induced hypertension. The cortocoid receptor I of Fisher 344 rats had a lower affinity than that of salt sensitive Wistar-Kyoto controls, as well as spontaneously hypertensive rats and Sprague-Dawley rats. In the present study, we have used DEAE-cellulose ion exchange chromatography to compare the structure (charge properties) and steroid specificity of vascular corticoid receptor I and II sites in these same rat hypertension models. No variations in ion exchange properties of type I and II receptors were found. Together with the lower aldosterone affinity of corticoid receptor I sites in Fischer 344 rats these data suggest an altered binding domain which is not seen as a difference in charge density of the receptor protein by ion exchange chromatography.

Original languageEnglish (US)
Pages (from-to)1231-1236
Number of pages6
JournalLife Sciences
Volume39
Issue number14
DOIs
StatePublished - Oct 6 1986

Fingerprint

Rats
Mineralocorticoid Receptors
Hypertension
Salts
Inbred F344 Rats
Ion Exchange Chromatography
Aldosterone
Ion exchange
Steroids
Chromatography
DEAE-Cellulose Chromatography
Mineralocorticoids
Ion Exchange
Genetic Models
Glucocorticoid Receptors
Inbred SHR Rats
DEAE-Cellulose
Smooth Muscle Myocytes
Blood Vessels
Sprague Dawley Rats

ASJC Scopus subject areas

  • Pharmacology

Cite this

A physico-chemical comparison of aortic receptors in rat hypertension models. / Meyer, Walter; Nichols, Nancy R.; Nguyen, Hanh H.

In: Life Sciences, Vol. 39, No. 14, 06.10.1986, p. 1231-1236.

Research output: Contribution to journalArticle

Meyer, Walter ; Nichols, Nancy R. ; Nguyen, Hanh H. / A physico-chemical comparison of aortic receptors in rat hypertension models. In: Life Sciences. 1986 ; Vol. 39, No. 14. pp. 1231-1236.
@article{5fd637a034a14343b7e6e8c97238b4dc,
title = "A physico-chemical comparison of aortic receptors in rat hypertension models",
abstract = "Rat models of genetic hypertension include spontaneous hypertension and resistance or sensitivity to mineralocorticoid and salt induced hypertension. Previously, altered aldosterone binding to corticoid receptor I was found in aortic smooth muscle cells cultured from Fischer 344 rats which are extremely resistant to steroid and salt induced hypertension. The cortocoid receptor I of Fisher 344 rats had a lower affinity than that of salt sensitive Wistar-Kyoto controls, as well as spontaneously hypertensive rats and Sprague-Dawley rats. In the present study, we have used DEAE-cellulose ion exchange chromatography to compare the structure (charge properties) and steroid specificity of vascular corticoid receptor I and II sites in these same rat hypertension models. No variations in ion exchange properties of type I and II receptors were found. Together with the lower aldosterone affinity of corticoid receptor I sites in Fischer 344 rats these data suggest an altered binding domain which is not seen as a difference in charge density of the receptor protein by ion exchange chromatography.",
author = "Walter Meyer and Nichols, {Nancy R.} and Nguyen, {Hanh H.}",
year = "1986",
month = "10",
day = "6",
doi = "10.1016/0024-3205(86)90183-9",
language = "English (US)",
volume = "39",
pages = "1231--1236",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "14",

}

TY - JOUR

T1 - A physico-chemical comparison of aortic receptors in rat hypertension models

AU - Meyer, Walter

AU - Nichols, Nancy R.

AU - Nguyen, Hanh H.

PY - 1986/10/6

Y1 - 1986/10/6

N2 - Rat models of genetic hypertension include spontaneous hypertension and resistance or sensitivity to mineralocorticoid and salt induced hypertension. Previously, altered aldosterone binding to corticoid receptor I was found in aortic smooth muscle cells cultured from Fischer 344 rats which are extremely resistant to steroid and salt induced hypertension. The cortocoid receptor I of Fisher 344 rats had a lower affinity than that of salt sensitive Wistar-Kyoto controls, as well as spontaneously hypertensive rats and Sprague-Dawley rats. In the present study, we have used DEAE-cellulose ion exchange chromatography to compare the structure (charge properties) and steroid specificity of vascular corticoid receptor I and II sites in these same rat hypertension models. No variations in ion exchange properties of type I and II receptors were found. Together with the lower aldosterone affinity of corticoid receptor I sites in Fischer 344 rats these data suggest an altered binding domain which is not seen as a difference in charge density of the receptor protein by ion exchange chromatography.

AB - Rat models of genetic hypertension include spontaneous hypertension and resistance or sensitivity to mineralocorticoid and salt induced hypertension. Previously, altered aldosterone binding to corticoid receptor I was found in aortic smooth muscle cells cultured from Fischer 344 rats which are extremely resistant to steroid and salt induced hypertension. The cortocoid receptor I of Fisher 344 rats had a lower affinity than that of salt sensitive Wistar-Kyoto controls, as well as spontaneously hypertensive rats and Sprague-Dawley rats. In the present study, we have used DEAE-cellulose ion exchange chromatography to compare the structure (charge properties) and steroid specificity of vascular corticoid receptor I and II sites in these same rat hypertension models. No variations in ion exchange properties of type I and II receptors were found. Together with the lower aldosterone affinity of corticoid receptor I sites in Fischer 344 rats these data suggest an altered binding domain which is not seen as a difference in charge density of the receptor protein by ion exchange chromatography.

UR - http://www.scopus.com/inward/record.url?scp=0022514444&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022514444&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(86)90183-9

DO - 10.1016/0024-3205(86)90183-9

M3 - Article

VL - 39

SP - 1231

EP - 1236

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 14

ER -