A positive role of Patched-Smoothened signaling in promoting cell proliferation during normal head development in Drosophila

Baragur V. Shyamala, Krishna M. Bhat

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The transmembrane receptor Patched regulates several developmental processes in both invertebrates and vertebrates. In vertebrates, Patched also acts as a tumor suppressor. The Patched pathway normally operates by negatively regulating Smoothened, a G-protein-coupled receptor; binding of Hedgehog ligand to Patched relieves this negative interaction and allows signaling by Smoothened. We show that Ptc regulates Drosophila head development by promoting cell proliferation in the eye-antennal disc. During head morphogenesis, Patched positively interacts with Smoothened, which leads to the activation of Activin type I receptor Baboon and stimulation of cell proliferation in the eye-antennal disc. Thus, loss of Ptc or Smoothened activity affects cell proliferation in the eye-antennal disc and results in adult head capsule defects. Similarly, reducing the dose of smoothened in a patched background enhances the head defects. Consistent with these results, gain-of-function Hedgehog interferes with the activation of Baboon by Patched and Smoothened, leading to a similar head capsule defect. Expression of an activated form of Baboon in the patched domain in a patched mutant background completely rescues the head defects. These results provide insight into head morphogenesis, a process we know very little about, and reveal an unexpected non-canonical positive signaling pathway in which Patched and Smoothened function to promote cell proliferation as opposed to repressing it.

Original languageEnglish (US)
Pages (from-to)1839-1847
Number of pages9
JournalDevelopment
Volume129
Issue number8
StatePublished - 2002
Externally publishedYes

Fingerprint

Drosophila
Head
Cell Proliferation
Papio
Hedgehogs
Morphogenesis
Vertebrates
Type I Activin Receptors
Invertebrates
G-Protein-Coupled Receptors
Ligands
Neoplasms

Keywords

  • Drosophila
  • Head
  • Patched

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

A positive role of Patched-Smoothened signaling in promoting cell proliferation during normal head development in Drosophila. / Shyamala, Baragur V.; Bhat, Krishna M.

In: Development, Vol. 129, No. 8, 2002, p. 1839-1847.

Research output: Contribution to journalArticle

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