A positively selected mutation in the WNV 2K peptide confers resistance to superinfection exclusion in vivo

Corey L. Campbell, Darci R. Smith, Irma Sanchez-Vargas, Bo Zhang, Pei-Yong Shi, Gregory D. Ebel

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Molecular epidemiologic studies of North American (NA) West Nile virus (WNV; Flaviviridae, Flavivirus) have documented the displacement of the introduced NY99 genotype with WN02. In addition, these studies have shown that particular substitutions are under positive selection. One occurs in the C-terminus of the NS4A coding sequence and results in a valine to methionine substitution at position nine of the 2K peptide. 2K-V9M confers the ability to overcome superinfection exclusion in vitro. We hypothesized that WNV strains bearing 2K-V9M have higher fitness than wildtype in Culex quinquefasciatus mosquitoes. Although infection rates and viral titers were not significantly different, virus dissemination rates were significantly higher with WNV 2K-V9M. As a super-infecting virus, WNV 2K-V9M was more successful than wildtype, however, in a mixed infection, 2K-V9M was not. These data support observations that 2K-V9M confers a context-specific selective advantage in mosquitoes and provides an in vivo mechanism for its positive selection.

Original languageEnglish (US)
Pages (from-to)228-232
Number of pages5
JournalVirology
Volume464-465
Issue number1
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Superinfection
Culicidae
Flaviviridae
Viruses
Flavivirus
West Nile virus
Peptides
Culex
Mutation
Valine
Virus Diseases
Coinfection
Methionine
Epidemiologic Studies
Genotype
In Vitro Techniques

Keywords

  • Flavivirus
  • Superinfection exclusion
  • Vector mosquito

ASJC Scopus subject areas

  • Virology
  • Medicine(all)

Cite this

A positively selected mutation in the WNV 2K peptide confers resistance to superinfection exclusion in vivo. / Campbell, Corey L.; Smith, Darci R.; Sanchez-Vargas, Irma; Zhang, Bo; Shi, Pei-Yong; Ebel, Gregory D.

In: Virology, Vol. 464-465, No. 1, 2014, p. 228-232.

Research output: Contribution to journalArticle

Campbell, Corey L. ; Smith, Darci R. ; Sanchez-Vargas, Irma ; Zhang, Bo ; Shi, Pei-Yong ; Ebel, Gregory D. / A positively selected mutation in the WNV 2K peptide confers resistance to superinfection exclusion in vivo. In: Virology. 2014 ; Vol. 464-465, No. 1. pp. 228-232.
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