A post-entry role for CD63 in early HIV-1 replication

Guangyu Li, Natallia Dziuba, Brian Friedrich, James L. Murray, Monique Ferguson

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Macrophages and CD4 + lymphocytes are the major reservoirs for HIV-1 infection. CD63 is a tetraspanin transmembrane protein, which has been shown to play an essential role during HIV-1 replication in macrophages. In this study, we further confirm the requirement of CD63 in early HIV-1 replication events in both macrophages and a CD4 + cell line. Further analysis revealed that viral attachment and cell-cell fusion were unaffected by CD63 silencing. However, CD63-depleted macrophages showed a significant decrease in the initiation and completion of HIV-1 reverse transcription, affecting subsequent events of the HIV-1 life cycle. Integration of HIV-1 cDNA as well as the formation of 2-LTR circles was notably reduced. Reporter assays showed that CD63 down regulation reduced production of the early HIV protein Tat. In agreement, CD63 silencing also inhibited production of the late protein p24. These findings suggest that CD63 plays an early post-entry role prior to or at the reverse transcription step.

Original languageEnglish (US)
Pages (from-to)315-324
Number of pages10
JournalVirology
Volume412
Issue number2
DOIs
StatePublished - Apr 10 2011

Fingerprint

HIV-1
Macrophages
Reverse Transcription
Human Immunodeficiency Virus Proteins
Cell Fusion
Life Cycle Stages
HIV Infections
Proteins
Down-Regulation
Complementary DNA
Lymphocytes
Cell Line

Keywords

  • CD63
  • HIV-1
  • Life cycle
  • Macrophage
  • SiRNA
  • Tetraspanin

ASJC Scopus subject areas

  • Virology

Cite this

A post-entry role for CD63 in early HIV-1 replication. / Li, Guangyu; Dziuba, Natallia; Friedrich, Brian; Murray, James L.; Ferguson, Monique.

In: Virology, Vol. 412, No. 2, 10.04.2011, p. 315-324.

Research output: Contribution to journalArticle

Li, G, Dziuba, N, Friedrich, B, Murray, JL & Ferguson, M 2011, 'A post-entry role for CD63 in early HIV-1 replication', Virology, vol. 412, no. 2, pp. 315-324. https://doi.org/10.1016/j.virol.2011.01.017
Li, Guangyu ; Dziuba, Natallia ; Friedrich, Brian ; Murray, James L. ; Ferguson, Monique. / A post-entry role for CD63 in early HIV-1 replication. In: Virology. 2011 ; Vol. 412, No. 2. pp. 315-324.
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