A potential basis for suppressed inflammatory cell function in pediatric cholestatic hosts

Patrick Roughneen, A. D. Kulkarni, R. J. Andrassy, B. J. Rowlands

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Infective mortality is common in children who have hepatic failure. We have demonstrated that experimental hepatic failure (EHF) profoundly suppresses T cell function in vivo. To determine the basis for immune suppression in EHF we postulated that this phenomenon is attributable to alterations in accessory macrophage (Ma) function, T cell subsets, interleukin-2 (IL-2) production, or serum inhibition. Wistar Furth rats (200 g) were randomized to EHF (n = 23), Sham (n = 23), and normal control (NC) (n = 23) groups. On day 21, splenocytes and sera were harvested and immune assays performed in vitro. Following are the results (mean ± SEM; Student's t test). Serum bilirubin was elevated in EHF versus Sham and NC groups (P <.01). EHF splenic macrophages suppressed PHA when added to microcultures at 105 concentration (-140 ± 550 v 12,263 ± 2,492 [Sham] and 21,413 ± 1,702 [NC] P <.01). This effect was not evident when macrophages were added back to microcultures at 103 and 104 concentrations, suggesting a dose-dependent inhibitory effect. T helper: suppressor ratios did not differ in EHF (1.3 ± 0.2) compared with Sham (1.4 ± 0.2) and NC groups (1.2 ± 0.1). IL-2 production was similar in EHF, Sham, and NC animals (112,141 ± 5,232 versus 106,691 ± 1,419 and 120,759 ± 3,249 counts per minute). T cell inhibitory activity was not demonstrable in EHF sera. These data show that splenic macrophages can inhibit T cell function in vitro. This phenomenon may be paramount in predisposing children with liver disease to infection.

Original languageEnglish (US)
Pages (from-to)204-207
Number of pages4
JournalJournal of Pediatric Surgery
Volume25
Issue number2
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Liver Failure
Pediatrics
Macrophages
Serum
T-Lymphocytes
Interleukin-2
Inbred WF Rats
Control Groups
T-Lymphocyte Subsets
Bilirubin
Liver Diseases
Students
Mortality
Infection

Keywords

  • Hepatic failure
  • infection

ASJC Scopus subject areas

  • Surgery

Cite this

A potential basis for suppressed inflammatory cell function in pediatric cholestatic hosts. / Roughneen, Patrick; Kulkarni, A. D.; Andrassy, R. J.; Rowlands, B. J.

In: Journal of Pediatric Surgery, Vol. 25, No. 2, 1990, p. 204-207.

Research output: Contribution to journalArticle

Roughneen, Patrick ; Kulkarni, A. D. ; Andrassy, R. J. ; Rowlands, B. J. / A potential basis for suppressed inflammatory cell function in pediatric cholestatic hosts. In: Journal of Pediatric Surgery. 1990 ; Vol. 25, No. 2. pp. 204-207.
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abstract = "Infective mortality is common in children who have hepatic failure. We have demonstrated that experimental hepatic failure (EHF) profoundly suppresses T cell function in vivo. To determine the basis for immune suppression in EHF we postulated that this phenomenon is attributable to alterations in accessory macrophage (Ma) function, T cell subsets, interleukin-2 (IL-2) production, or serum inhibition. Wistar Furth rats (200 g) were randomized to EHF (n = 23), Sham (n = 23), and normal control (NC) (n = 23) groups. On day 21, splenocytes and sera were harvested and immune assays performed in vitro. Following are the results (mean ± SEM; Student's t test). Serum bilirubin was elevated in EHF versus Sham and NC groups (P <.01). EHF splenic macrophages suppressed PHA when added to microcultures at 105 concentration (-140 ± 550 v 12,263 ± 2,492 [Sham] and 21,413 ± 1,702 [NC] P <.01). This effect was not evident when macrophages were added back to microcultures at 103 and 104 concentrations, suggesting a dose-dependent inhibitory effect. T helper: suppressor ratios did not differ in EHF (1.3 ± 0.2) compared with Sham (1.4 ± 0.2) and NC groups (1.2 ± 0.1). IL-2 production was similar in EHF, Sham, and NC animals (112,141 ± 5,232 versus 106,691 ± 1,419 and 120,759 ± 3,249 counts per minute). T cell inhibitory activity was not demonstrable in EHF sera. These data show that splenic macrophages can inhibit T cell function in vitro. This phenomenon may be paramount in predisposing children with liver disease to infection.",
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