A practical tissue sampling method using ordinary paper for molecular detection of infectious bursal disease virus RNA by RT-PCR

Min Thein Maw, Tsuyoshi Yamaguchi, Christopher J. Kasanga, Kaori Terasaki, Hideto Fukushi

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

A practical sampling method for bursal tissue using ordinary paper for molecular diagnosis of infectious bursal disease (IBD) was established. IBD virus-infected bursa was directly smeared on chromatography paper, filter paper, or stationery copy paper and was then fixed with absolute ethanol, Tris-HCl-saturated phenol, or phenol:chloroform:isoamyl alcohol (25:24:1). Flinders Technology Associates (FTA®) card, which is designed for the collection of biological samples for molecular detection, was also used. After storage at 37 C for up to 30 days, total RNA directly extracted from the tissue fixed on the papers and FTA® card were subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) for detection of IBD virus (IBDV) RNA. In addition, the ability of each chemical used in the fixation and the FTA® card to inactivate IBDV was evaluated. Regardless of the paper quality, storage period, and fixation method, IBDV RNA was consistently detected in all of the samples. IBDV in the bursal tissue was inactivated with phenol but not with ethanol or the unknown chemicals in FTA® card. These results show that ordinary papers sustain the viral RNA, as does FTA® card, but phenol fixation is superior to FTA® card in inactivating IBDV. The new sampling method using ordinary paper with phenol fixation is safe, inexpensive, simple, and easy, and is thus suitable for conducting a global survey of IBD even where laboratory resources are limited. This practical method should contribute to the control of IBD worldwide.

Original languageEnglish (US)
Pages (from-to)556-560
Number of pages5
JournalAvian Diseases
Volume50
Issue number4
DOIs
StatePublished - Dec 1 2006
Externally publishedYes

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Keywords

  • Diagnosis
  • Infectious bursal disease virus
  • Ordinary paper
  • Reverse transcriptase- polymerase chain reaction

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Immunology and Microbiology(all)

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