A proteomics analysis of the effects of chronic hemiparetic stroke on troponin T expression in human Vastus lateralis

Jeffrey P. Rabek, Charlene E. Hafer-Macko, James K. Amaning, James H. DeFord, Vincent L. Dimayuga, Mark A. Madsen, Richard F. Macko, John Papaconstantinou

Research output: Contribution to journalArticle

Abstract

Stroke disability is attributed to upper motor neuron deficits resulting from ischemic brain injury. We have developed proteome maps of the Vastus lateralis to examine the effects of ischemic brain injury on paretic skeletal muscle myofilament proteins. Proteomics analyses from seven hemiparetic stroke patients have detected a decrease of three troponin T isoforms in the paretic muscle suggesting that myosin-actin interactions may be attenuated. We propose that ischemic brain injury may prevent troponin T participation in complex formation thereby affecting the protein interactions associated with excitation-contraction coupling. We have also detected a novel skeletal troponin T isoform that has a C-terminal variation. Our data suggest that the decreased slow troponin T isoform pools in the paretic limb may contribute to the gait deficit after stroke. The complexity of the neurological deficit on Vastus lateralis is suggested by the multiple changes in proteins detected by our proteomics mapping.

Original languageEnglish (US)
Pages (from-to)839-849
Number of pages11
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume64
Issue number8
DOIs
StatePublished - Aug 1 2009

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Keywords

  • 2D gel electrophoresis
  • Skeletal muscle proteome
  • Stroke
  • Troponin T isoforms

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology

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